Lox'd in translation: contradictions in the nomenclature surrounding common lox-site mutants and their implications in experiments
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- dc.contributor.author Shaw, Daniel, 1993-
- dc.contributor.author Serrano Pubull, Luis, 1982-
- dc.contributor.author Lluch-Senar, Maria 1982-
- dc.date.accessioned 2021-03-22T12:14:08Z
- dc.date.available 2021-03-22T12:14:08Z
- dc.date.issued 2021
- dc.description.abstract The Cre-Lox system is a highly versatile and powerful DNA recombinase mechanism, mainly used in genetic engineering to insert or remove desired DNA sequences. It is widely utilized across multiple fields of biology, with applications ranging from plants, to mammals, to microbes. A key feature of this system is its ability to allow recombination between mutant lox sites. Two of the most commonly used mutant sites are named lox66 and lox71, which recombine to create a functionally inactive double mutant lox72 site. However, a large portion of the published literature has incorrectly annotated these mutant lox sites, which in turn can lead to difficulties in replication of methods, design of proper vectors and confusion over the proper nomenclature. Here, we demonstrate common errors in annotations, the impacts they can have on experimental viability, and a standardized naming convention. We also show an example of how this incorrect annotation can induce toxic effects in bacteria that lack optimal DNA repair systems, exemplified by Mycoplasma pneumoniae.
- dc.description.sponsorship This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement 634942 (MycoSynVac) and was also financed by the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme, under grant agreement 670216 (MYCOCHASSIS). We also acknowledge support of the Spanish Ministry of Economy, Industry and Competitiveness (MEIC) to the EMBL partnership, the Centro de Excelencia Severo Ochoa and the CERCA Programme/Generalitat de Catalunya
- dc.format.mimetype application/pdf
- dc.identifier.citation Shaw D, Serrano L, Lluch-Senar M. Lox'd in translation: contradictions in the nomenclature surrounding common lox-site mutants and their implications in experiments. Microbiology (Reading). 2021 Jan;167(1). DOI: 10.1099/mic.0.000997
- dc.identifier.doi http://dx.doi.org/10.1099/mic.0.000997
- dc.identifier.issn 1350-0872
- dc.identifier.uri http://hdl.handle.net/10230/46885
- dc.language.iso eng
- dc.publisher Microbiology society
- dc.relation.ispartof Microbiology (Reading). 2021 Jan;167(1)
- dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/634942
- dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/670216
- dc.rights © 2021 Daniel Shaw, Luis Serrano, Maria Lluch-Senar. This is an open-access article distributed under the terms of the Creative Commons Attribution License
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri https://creativecommons.org/licenses/by/4.0/deed.ast
- dc.subject.other Enginyeria genètica
- dc.subject.other Seqüència de nucleòtids
- dc.subject.other Llocs loxP
- dc.title Lox'd in translation: contradictions in the nomenclature surrounding common lox-site mutants and their implications in experiments
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion