Generation of induced pluripotent stem cells from human cord blood cells with only two factors:OCT4 and SOX2

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• dc.contributor.author Giorgetti, Alessandraca
• dc.contributor.author Montserrat Pulido, Núria
• dc.contributor.author Rodríguez Pizà, Ignasi
• dc.contributor.author Azqueta, Carmen
• dc.contributor.author Veiga, Anna
• dc.contributor.author Izpisúa Belmonte, J. C.
• dc.date.accessioned 2011-07-27T08:28:05Z
• dc.date.available 2011-07-27T08:28:05Z
• dc.date.issued 2010ca
• dc.description.abstract Induced pluripotent stem cells (iPSC ) provide an invaluable resource for regenerative medicine as they allow the generation/nof patient-specific progenitors with potential value for cell therapy. However, in many instances, an off-the-shelf approach is/ndesirable, such as for cell therapy of acute conditions or when the patient’s somatic cells are altered as a consequence of a chronic/ndisease or aging. Cord blood (CB) stem cells appear ideally suited for this purpose as they are young cells expected to carry/nminimal somatic mutations and possess the immunological immaturity of newborn cells; additionally, several hundred thousand/nimmunotyped CB units are readily available through a worldwide network of CB banks. Here we present a detailed protocol for the/nderivation of CB stem cells and how they can be reprogrammed to pluripotency by retroviral transduction with only two factors/n(OCT 4 and SO X2) in 2 weeks and without the need for additional chemical compounds.
• dc.format.mimetype application/pdfca
• dc.identifier.citation Giorgetti A, Montserrat N, Rodriguez-Piza I, Azqueta C, Veiga A, Izpisúa JC. Generation of induced pluripotent stem cells from human cord blood cells with only two factors: Oct4 and Sox2. Nat Protoc. 2010;5(4):811-20. DOI: 10.1038/nprot.2010.16
• dc.identifier.doi http://dx.doi.org/10.1038/nprot.2010.16
• dc.identifier.issn 1754-2189
• dc.identifier.uri http://hdl.handle.net/10230/12432
• dc.language.iso engca
• dc.publisher Nature Publishing Groupca
• dc.relation.ispartof Nature Protocols. 2010;5(4):811-20
• dc.rights © Nature Publishing Groupca
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.subject.other Cél·lules mare embrionàries
• dc.subject.other Medicina regenerativa
• dc.title Generation of induced pluripotent stem cells from human cord blood cells with only two factors:OCT4 and SOX2ca
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/publishedVersion