Three-dimensional stem cell models of mammalian gastrulation
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- dc.contributor.author Turner, David A.
- dc.contributor.author Martínez Arias, Alfonso
- dc.date.accessioned 2025-03-27T06:45:42Z
- dc.date.available 2025-03-27T06:45:42Z
- dc.date.issued 2024
- dc.description.abstract Gastrulation is a key milestone in the development of an organism. It is a period of cell proliferation and coordinated cellular rearrangement, that creates an outline of the body plan. Our current understanding of mammalian gastrulation has been improved by embryo culture, but there are still many open questions that are difficult to address because of the intrauterine development of the embryos and the low number of specimens. In the case of humans, there are additional difficulties associated with technical and ethical challenges. Over the last few years, pluripotent stem cell models are being developed that have the potential to become useful tools to understand the mammalian gastrulation. Here we review these models with a special emphasis on gastruloids and provide a survey of the methods to produce them robustly, their uses, relationship to embryos, and their prospects as well as their limitations.
- dc.description.sponsorship We want to thank Naomi Moris and members of our research groups for discussions that have sharpened the manuscript. A.M.A. is funded by an ERC AdG (MiniEmbryoBlueprint_ 834580) and the “Maria de Maeztu” Programme for Units of Excellence in R&D (Grant No. CEX2018-000792-M) and is an inventor in two patents on Human Polarised Three-dimensional Cellular Aggregates PCT/GB2019/052670 and Polarised Three-dimensional Cellular Aggregates PCT/GB2019/052668. D.A.T. is funded by grants from the BBSRC (BB/X000907/1, BB/Y00311X/1) and the NC3Rs (NC/T002131/1, NC/X001938/1).
- dc.format.mimetype application/pdf
- dc.identifier.citation Turner DA, Martinez Arias A. Three-dimensional stem cell models of mammalian gastrulation. Bioessays. 2024 Dec;46(12):e2400123. DOI: 10.1002/bies.202400123
- dc.identifier.doi http://dx.doi.org/10.1002/bies.202400123
- dc.identifier.issn 0265-9247
- dc.identifier.uri http://hdl.handle.net/10230/70019
- dc.language.iso eng
- dc.publisher Wiley
- dc.relation.ispartof Bioessays. 2024 Dec;46(12):e2400123
- dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/834580
- dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/CEX2018-000792-M
- dc.rights © 2024 The Author(s). BioEssays published by Wiley Periodicals LLC. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by/4.0/
- dc.subject.keyword 3D models
- dc.subject.keyword Embryonic stem cells
- dc.subject.keyword Gastrulation
- dc.subject.keyword Gastruloids
- dc.subject.keyword Organoids
- dc.title Three-dimensional stem cell models of mammalian gastrulation
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion