Enhanced susceptibility to tomato chlorosis virus (ToCV) in Hsp90- and Sgt1-silenced pPlants: insights from gene expression dynamics

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• dc.contributor.author Ontiveros, Irene
• dc.contributor.author Fernández-Pozo, Noé
• dc.contributor.author Esteve-Codina, Anna
• dc.contributor.author López-Moya, Juan José
• dc.contributor.author Díaz Pendón, Juan Antonio
• dc.date.accessioned 2024-02-08T07:10:01Z
• dc.date.available 2024-02-08T07:10:01Z
• dc.date.issued 2023
• dc.description.abstract The emerging whitefly-transmitted crinivirus tomato chlorosis virus (ToCV) causes substantial economic losses by inducing yellow leaf disorder in tomato crops. This study explores potential resistance mechanisms by examining early-stage molecular responses to ToCV. A time-course transcriptome analysis compared naïve, mock, and ToCV-infected plants at 2, 7, and 14 days post-infection (dpi). Gene expression changes were most notable at 2 and 14 dpi, likely corresponding to whitefly feeding and viral infection. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed key genes and pathways associated with ToCV infection, including those related to plant immunity, flavonoid and steroid biosynthesis, photosynthesis, and hormone signaling. Additionally, virus-derived small interfering RNAs (vsRNAs) originating from ToCV predominantly came from RNA2 and were 22 nucleotides in length. Furthermore, two genes involved in plant immunity, Hsp90 (heat shock protein 90) and its co-chaperone Sgt1 (suppressor of the G2 allele of Skp1) were targeted through viral-induced gene silencing (VIGS), showing a potential contribution to basal resistance against viral infections since their reduction correlated with increased ToCV accumulation. This study provides insights into tomato plant responses to ToCV, with potential implications for developing effective disease control strategies.
• dc.description.sponsorship Research in our laboratories has received funding from projects PID2019-105692RB-I00 and PID2019-107657RB-C21 funded by MCIN/AEI/10.13039/501100011033, and PID2022-139376OB-C32 and PID2022-139376OB-C33 funded by MCIN/AEI/10.13039/501100011033 by “ERDF A way of making Europe”. This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No 101000570. I.O. was the recipient of FPI grant BES-2017-080808 funded by MCIN/AEI/10.13039/501100011033 and by “ESF Investing in your Future”. N.F-P was the recipient of the Spanish Ramón y Cajal Grant RYC2020-030219-I funded by MCIN/AEI/10.13039/501100011033 and by “ESF Investing in your Future”. Research at CRAG was also supported by grants SEV-2015-0533 and CEX2019-000902-S funded by MCIN/AEI/10.13039/501100011033, and by the CERCA Program, Generalitat de Catalunya.
• dc.format.mimetype application/pdf
• dc.identifier.citation Ontiveros I, Fernández-Pozo N, Esteve-Codina A, López-Moya JJ, Díaz-Pendón JA. Enhanced susceptibility to tomato chlorosis virus (ToCV) in Hsp90- and Sgt1-silenced pPlants: insights from gene expression dynamics. Viruses. 2023 Nov 30;15(12):2370. DOI: 10.3390/v15122370
• dc.identifier.doi http://dx.doi.org/10.3390/v15122370
• dc.identifier.issn 1999-4915
• dc.identifier.uri http://hdl.handle.net/10230/58993
• dc.language.iso eng
• dc.publisher MDPI
• dc.relation.ispartof Viruses. 2023 Nov 30;15(12):2370
• dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/101000570
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PID2019-105692RB-I00
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PID2019-107657RB-C21
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PE/PID2022-139376OB-C32
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PE/PID2022-139376OB-C33
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/BES-2017-080808
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/RYC2020-030219-I
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/CEX2019-000902-S
• dc.rights © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Bemisia tabaci
• dc.subject.keyword Hsp90
• dc.subject.keyword Sgt1
• dc.subject.keyword ToCV
• dc.subject.keyword Basal resistance
• dc.subject.keyword Tomato
• dc.title Enhanced susceptibility to tomato chlorosis virus (ToCV) in Hsp90- and Sgt1-silenced pPlants: insights from gene expression dynamics
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion