Sperm physiology and in vitro fertilising ability rely on basal metabolic activity: insights from the pig model

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• dc.contributor.author Mateo-Otero, Yentel
• dc.contributor.author Madrid-Gambin, Francisco
• dc.contributor.author Llavanera, Marc
• dc.contributor.author Gomez-Gomez, Àlex
• dc.contributor.author Haro, Noemí
• dc.contributor.author Pozo Mendoza, Óscar J., 1975-
• dc.contributor.author Yeste, Marc
• dc.date.accessioned 2024-02-29T06:48:33Z
• dc.date.available 2024-02-29T06:48:33Z
• dc.date.issued 2023
• dc.description.abstract Whether basal metabolic activity in sperm has any influence on their fertilising capacity has not been explored. Using the pig as a model, the present study investigated the relationship of energetic metabolism with sperm quality and function (assessed through computer-assisted sperm analysis and flow cytometry), and fertility (in vitro fertilisation (IVF) outcomes). In semen samples from 16 boars, levels of metabolites related to glycolysis, ketogenesis and Krebs cycle were determined through a targeted metabolomics approach using liquid chromatography-tandem mass spectrometry. High-quality sperm are associated to greater levels of glycolysis-derived metabolites, and oocyte fertilisation and embryo development are conditioned by the sperm metabolic status. Interestingly, glycolysis appears to be the preferred catabolic pathway of the sperm giving rise to greater percentages of embryos at day 6. In conclusion, this study shows that the basal metabolic activity of sperm influences their function, even beyond fertilisation.
• dc.description.sponsorship The authors would like to thank Frigorífics Costa Brava (Riudellots de la Selva, Girona) for supplying ovaries. This study was funded by the Ministry of Science and Innovation, Spain (AGL2017-88329-R, FJC2018-035791-I, FPU18/00666 and PID2020-113320RB-I00), the Regional Government of Catalonia, Spain (2017-SGR-1229 and 2020-FI-B-00412), and the Catalan Institution for Research and Advanced Studies (ICREA).
• dc.format.mimetype application/pdf
• dc.identifier.citation Mateo-Otero Y, Madrid-Gambin F, Llavanera M, Gomez-Gomez A, Haro N, Pozo OJ, Yeste M. Sperm physiology and in vitro fertilising ability rely on basal metabolic activity: insights from the pig model. Commun Biol. 2023 Mar 30;6(1):344. DOI: 10.1038/s42003-023-04715-3
• dc.identifier.doi http://dx.doi.org/10.1038/s42003-023-04715-3
• dc.identifier.issn 2399-3642
• dc.identifier.uri http://hdl.handle.net/10230/59291
• dc.language.iso eng
• dc.publisher Nature Research
• dc.relation.ispartof Commun Biol. 2023 Mar 30;6(1):344
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/AGL2017-88329-R
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/FJC2018-035791-I
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PID2020-113320RB-I00
• dc.rights © The Author(s) 2023. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Cell biology
• dc.subject.keyword Diagnostic markers
• dc.subject.keyword Metabolomics
• dc.title Sperm physiology and in vitro fertilising ability rely on basal metabolic activity: insights from the pig model
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion