Mapping of six somatic linker histone H1 variants in human breast cancer cells uncovers specific features of H1.2

Millán Ariño, Lluís, 1984-; Islam, Abul, 1978-; Izquierdo Bouldstridge, Andrea; Mayor, Regina; Terme, Jean Michel; Luque, Neus; Sancho Medina, Mónica; López Bigas, Núria; Jordan Vallès, Albert

Mapping of six somatic linker histone H1 variants in human breast cancer cells uncovers specific features of H1.2

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dc.contributor.author Millán Ariño, Lluís, 1984-ca
dc.contributor.author Islam, Abul, 1978-ca
dc.contributor.author Izquierdo Bouldstridge, Andreaca
dc.contributor.author Mayor, Reginaca
dc.contributor.author Terme, Jean Michelca
dc.contributor.author Luque, Neusca
dc.contributor.author Sancho Medina, Mónicaca
dc.contributor.author López Bigas, Núriaca
dc.contributor.author Jordan Vallès, Albertca
dc.date.accessioned 2015-04-07T09:36:29Z
dc.date.available 2015-04-07T09:36:29Z
dc.date.issued 2014ca
dc.description.abstract Seven linker histone H1 variants are present in human somatic cells with distinct prevalence across cell types. Despite being key structural components of chromatin, it is not known whether the different variants have specific roles in the regulation of nuclear processes or are differentially distributed throughout the genome. Using variant-specific antibodies to H1 and hemagglutinin (HA)-tagged recombinant H1 variants expressed in breast cancer cells, we have investigated the distribution of six H1 variants in promoters and genome-wide. H1 is depleted at promoters depending on its transcriptional status and differs between variants. Notably, H1.2 is less abundant than other variants at the transcription start sites of inactive genes, and promoters enriched in H1.2 are different from those enriched in other variants and tend to be repressed. Additionally, H1.2 is enriched at chromosomal domains characterized by low guanine–cytosine (GC) content and is associated with lamina-associated domains. Meanwhile, other variants are associated with higher GC content, CpG islands and gene-rich domains. For instance, H1.0 and H1X are enriched at gene-rich chromosomes, whereas H1.2 is depleted. In short, histone H1 is not uniformly distributed along the genome and there are differences between variants, H1.2 being the one showing the most specific pattern and strongest correlation with low gene expression.en
dc.description.sponsorship Ministerio de Ciencia e Innovació n of Spain (MICINN) and FEDER [BFU2011-23057 to A.J.]; Ministerio de Economía y Competitividad [SAF2012-36199 to N.L.-B.]; Generalitat de Catalunya [2009-SGR-1222 to A.J.]; JAE-Doc contract from CSIC-MICINN (to J.-M.T.); TA contract from CSIC-MICINN (to R.M.); FPU predoctoral fellowship from MICINN (to L.M.-A.). Funding for open access charge: Ministerio de Ciencia e Innovación of Spain (MICINN) and FEDER [BFU2011-23057 to A.J.]en
dc.format.mimetype application/pdfca
dc.identifier.citation Millan-Arino L, Islam A, Izquierdo-Bouldstridge A, Mayor R, Terme JM, Luque N et al. Mapping of six somatic linker histone H1 variants in human breast cancer cells uncovers specific features of H1.2. Nucleic Acids Research. 2014;42(7):4474-93. DOI: 10.1093/nar/gku079ca
dc.identifier.doi http://dx.doi.org/10.1093/nar/gku079
dc.identifier.issn 0305-1048ca
dc.identifier.uri http://hdl.handle.net/10230/23347
dc.language.iso engca
dc.publisher Oxford University Pressca
dc.relation.ispartof Nucleic Acids Research. 2014;42(7):4474-93
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2011-23057
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/SAF2012-36199
dc.rights © Millan-Arino L, Islam A, Izquierdo-Bouldstridge A, Mayor R, Terme JM, Luque N, Sancho M, Lopez-Bigas N, Jordan A [2014]. Published by Oxford University Press. This is an Open Access article distributed under the terms of a Creative Commons Attribution Licenseca
dc.rights.accessRights info:eu-repo/semantics/openAccessca
dc.rights.uri http://creativecommons.org/licenses/by/3.0/
dc.subject.other Mama -- Càncer -- Aspectes genèticsca
dc.subject.other Histonesca
dc.subject.other Transcripció genèticaca
dc.title Mapping of six somatic linker histone H1 variants in human breast cancer cells uncovers specific features of H1.2en
dc.type info:eu-repo/semantics/articleca
dc.type.version info:eu-repo/semantics/publishedVersionca

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