Kinesin-8 and Dis1/TOG collaborate to limit spindle elongation from prophase to anaphase A for proper chromosome segregation in fission yeast
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- dc.contributor.author Pinder, Corinne
- dc.contributor.author Matsuo, Yuzy
- dc.contributor.author Maurer, Sebastian
- dc.contributor.author Toda, Takashi
- dc.date.accessioned 2020-03-30T11:30:57Z
- dc.date.available 2020-03-30T11:30:57Z
- dc.date.issued 2019
- dc.description.abstract High-fidelity chromosome segregation relies on proper microtubule regulation. Kinesin-8 has been shown to destabilise microtubules to reduce metaphase spindle length and chromosome movements in multiple species. XMAP215/chTOG polymerases catalyse microtubule growth for spindle assembly, elongation and kinetochore-microtubule attachment. Understanding of their biochemical activity has advanced, but little work directly addresses the functionality and interplay of these conserved factors. We utilised the synthetic lethality of fission yeast kinesin-8 (Klp5-Klp6) and XMAP215/chTOG (Dis1) to study their individual and overlapping roles. We found that the non-motor kinesin-8 tailbox is essential for mitotic function; mutation compromises plus-end-directed processivity. Klp5-Klp6 induces catastrophes to control microtubule length and, surprisingly, Dis1 collaborates with kinesin-8 to slow spindle elongation. Together, they enforce a maximum spindle length for a viable metaphase-anaphase transition and limit elongation during anaphase A to prevent lagging chromatids. Our work provides mechanistic insight into how kinesin-8 negatively regulates microtubules and how this functionally overlaps with Dis1 and highlights the importance of spindle length control in mitosis.
- dc.format.mimetype application/pdf
- dc.identifier.citation Pinder C, Matsuo Y, Maurer SP, Toda T. Kinesin-8 and Dis1/TOG collaborate to limit spindle elongation from prophase to anaphase A for proper chromosome segregation in fission yeast. J Cell Sci. 2019; 132(18). pii: jcs232306. DOI: 10.1242/jcs.232306
- dc.identifier.doi http://dx.doi.org/10.1242/jcs.232306
- dc.identifier.issn 0021-9533
- dc.identifier.uri http://hdl.handle.net/10230/44113
- dc.language.iso eng
- dc.publisher Company of Biologists
- dc.relation.ispartof J Cell Sci. 2019; 132(18). pii: jcs232306
- dc.rights © 2019. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri https://creativecommons.org/licenses/by/4.0
- dc.subject.keyword Fission yeast
- dc.subject.keyword Kinesin-8
- dc.subject.keyword Microtubules
- dc.subject.keyword Spindle elongation
- dc.subject.keyword XMAP215/TOG
- dc.title Kinesin-8 and Dis1/TOG collaborate to limit spindle elongation from prophase to anaphase A for proper chromosome segregation in fission yeast
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion