An experimental study of muscular injury repair in a mouse model of notexin-induced lesion with EPI® technique

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• dc.contributor.author Abat, Ferranca
• dc.contributor.author Valles, Soraya-Lca
• dc.contributor.author Gelber, Pablo-Eduardoca
• dc.contributor.author Polidori, Fernandoca
• dc.contributor.author Jorda, Adrianca
• dc.contributor.author García-Herreros, Sergioca
• dc.contributor.author Monllau García, Juan Carlosca
• dc.contributor.author Sanchez-Ibáñez, Jose-Manuelca
• dc.date.accessioned 2015-06-03T07:57:27Z
• dc.date.available 2015-06-03T07:57:27Z
• dc.date.issued 2015
• dc.description.abstract BACKGROUND: The mechanisms of muscle injury repair after EPI® technique, a treatment based on electrical stimulation, have not been described. This study determines whether EPI® therapy could improve muscle damage. METHODS: Twenty-four rats were divided into a control group, Notexin group (7 and 14 days) and a Notexin + EPI group. To induce muscle injury, Notexin was injected in the quadriceps of the left extremity of rats. Pro-inflammatory interleukin 1-beta (IL-1beta) and tumoral necrosis factor-alpha (TNF-alpha) were determined by ELISA. The expression of receptor peroxisome gamma proliferator activator (PPAR-gamma), vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptor-1 (VEGF-R1) were determined by western-blot. RESULTS: The plasma levels of TNF-alpha and IL-1beta in Notexin-injured rats showed a significant increase compared with the control group. EPI® produced a return of TNF-alpha and IL-1beta values to control levels. PPAR-gamma expression diminished injured quadriceps muscle in rats. EPI® increased PPAR-gamma, VEGF and VEGF-R1 expressions. EPI® decreased plasma levels of pro-inflammatory TNF-alpha and IL-1beta and increased anti-inflammatory PPAR-gamma and proangiogenic factors as well as VEGF and VEGF-R1 expressions. CONCLUSION: The EPI® technique may affect inflammatory mediators in damaged muscle tissue and influences the new vascularization of the injured area. These results suggest that EPI® might represent a useful new therapy for the treatment of muscle injuries. Although our study in rats may represent a valid approach to evaluate EPI® treatment, studies designed to determine how the EPI® treatment may affect recovery of injury in humans are needed.ca
• dc.format.mimetype application/pdfca
• dc.identifier.citation Abat F, Valles SL, Gelber PE, Polidori F, Jorda A, García-Herreros S. et al. An experimental study of muscular injury repair in a mouse model of notexin-induced lesion with EPI® technique. BMC Sports Sci Med Rehabil. 2015 Apr; 17(7):7. doi: 10.1186/s13102-015-0002-0. eCollection 2015.ca
• dc.identifier.doi http://dx.doi.org/10.1186/s13102-015-0002-0
• dc.identifier.issn 2052-1847
• dc.identifier.uri http://hdl.handle.net/10230/23719
• dc.language.iso engca
• dc.publisher BioMed Centralca
• dc.relation.ispartof BMC Sports Science, Medicine and Rehabilitation. 2015 Apr; 17(7):7
• dc.rights © 2015 Abat et al.; licensee BioMed Central. This is an Open Access article distributed under the terms of the Creative/nCommons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and/nreproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain/nDedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article,/nunless otherwise stateca
• dc.rights.accessRights info:eu-repo/semantics/openAccessca
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/ca
• dc.subject.other Músculs -- Malaltiesca
• dc.title An experimental study of muscular injury repair in a mouse model of notexin-induced lesion with EPI® techniqueca
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/publishedVersionca