An RNA polymerase III general transcription factor engages in cell type-specific chromatin looping

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• dc.contributor.author De Llobet, Lara Isabel
• dc.contributor.author Di Vona, Chiara, 1981-
• dc.contributor.author Morselli, Marco
• dc.contributor.author Vezzoli, Marco
• dc.contributor.author Montanini, Barbara
• dc.contributor.author Teichmann, Martin
• dc.contributor.author de la Luna, Susana
• dc.contributor.author Ferrari, Roberto
• dc.date.accessioned 2022-04-07T06:10:21Z
• dc.date.available 2022-04-07T06:10:21Z
• dc.date.issued 2022
• dc.description.abstract Transcription factors (TFs) bind DNA in a sequence-specific manner and are generally cell type-specific factors and/or developmental master regulators. In contrast, general TFs (GTFs) are part of very large protein complexes and serve for RNA polymerases' recruitment to promoter sequences, generally in a cell type-independent manner. Whereas, several TFs have been proven to serve as anchors for the 3D genome organization, the role of GTFs in genome architecture have not been carefully explored. Here, we used ChIP-seq and Hi-C data to depict the role of TFIIIC, one of the RNA polymerase III GTFs, in 3D genome organization. We find that TFIIIC genome occupancy mainly occurs at specific regions, which largely correspond to Alu elements; other characteristic classes of repetitive elements (REs) such as MIR, FLAM-C and ALR/alpha are also found depending on the cell's developmental origin. The analysis also shows that TFIIIC-enriched regions are involved in cell type-specific DNA looping, which does not depend on colocalization with the master architectural protein CTCF. This work extends previous knowledge on the role of TFIIIC as a bona fide genome organizer whose action participates in cell type-dependent 3D genome looping via binding to REs.
• dc.description.sponsorship This work was supported by Bando Galileo 2022 (G22-142) to R.F. and M.T. This work was also supported by the Spanish Ministry of Science and Innovation (PID2019-107185GB-I00) and Secretaria d’Universitats i Recerca del Departament d’Empresa i Coneixement de la Generalitat de Catalunya (2017SGR1163) to S.L. The CRG acknowledge the support of the Spanish Ministry of Science and Innovation to the EMBL partnership, the Centro de Excelencia Severo Ochoa and the support of the CERCA Programme/Generalitat de Catalunya
• dc.format.mimetype application/pdf
• dc.identifier.citation De Llobet LI, Di Vona C, Morselli M, Vezzoli M, Montanini B, Teichmann M et al. An RNA polymerase III general transcription factor engages in cell type-specific chromatin looping. Int J Mol Sci. 2022 Feb 18;23(4):2260. DOI: 10.3390/ijms23042260
• dc.identifier.doi http://dx.doi.org/10.3390/ijms23042260
• dc.identifier.issn 1661-6596
• dc.identifier.uri http://hdl.handle.net/10230/52839
• dc.language.iso eng
• dc.publisher MDPI
• dc.rights © 2022 by Lara de Llobet Cucalon et al. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/4.0/)
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri https://creativecommons.org/licenses/by/4.0/
• dc.subject.other Genètica
• dc.subject.other Genòmica
• dc.subject.other ADN
• dc.subject.other RNA
• dc.title An RNA polymerase III general transcription factor engages in cell type-specific chromatin looping
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion