A simple microfluidic platform to study age-dependent protein abundance and localization changes in Saccharomyces cerevisiae

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• dc.contributor.author Cabrera, Margarita
• dc.contributor.author Novarina, Daniele
• dc.contributor.author Rempel, Irina L.
• dc.contributor.author Veenhoff, Liesbeth M.
• dc.contributor.author Chang, Michael
• dc.date.accessioned 2023-06-06T06:24:35Z
• dc.date.available 2023-06-06T06:24:35Z
• dc.date.issued 2017
• dc.description.abstract The budding yeast Saccharomyces cerevisiae divides asymmetrically, with a smaller daughter cell emerging from its larger mother cell. While the daughter lineage is immortal, mother cells age with each cell division and have a finite lifespan. The replicative ageing of the yeast mother cell has been used as a model to study the ageing of mitotically active human cells. Several microfluidic platforms, which use fluid flow to selectively remove daughter cells, have recently been developed that can monitor cell physiology as mother cells age. However, these platforms are not trivial to set up and users often require many hours of training. In this study, we have developed a simple system, which combines a commercially available microfluidic platform (the CellASIC ONIX Microfluidic Platform) and a genetic tool to prevent the proliferation of daughter cells (the Mother Enrichment Program), to monitor protein abundance and localization changes during approximately the first half of the yeast replicative lifespan. We validated our system by observing known age-dependent changes, such as decreased Sir2 abundance, and have identified a protein with a previously unknown age-dependent change in localization.
• dc.format.mimetype application/pdf
• dc.identifier.citation Cabrera M, Novarina D, Rempel IL, Veenhoff LM, Chang M. A simple microfluidic platform to study age-dependent protein abundance and localization changes in Saccharomyces cerevisiae. Microb Cell. 2017 May;4(5):169-74. DOI: 10.15698/mic2017.05.573
• dc.identifier.doi http://dx.doi.org/10.15698/mic2017.05.573
• dc.identifier.issn 2311-2638
• dc.identifier.uri http://hdl.handle.net/10230/57068
• dc.language.iso eng
• dc.publisher Shared Science Publishers
• dc.relation.ispartof Microbial Cell. 2017 May;4(5):169-74
• dc.rights A simple microfluidic platform to study age-dependent protein abundance and localization changes in Saccharomyces cerevisiae by © 2017 Cabrera et al. is licensed under a Creative Commons Attribution 4.0 International License.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Yeast
• dc.subject.keyword Replicative ageing
• dc.subject.keyword Microfluidics
• dc.subject.keyword CellASIC
• dc.subject.keyword Protein abundance
• dc.subject.keyword Protein localization
• dc.title A simple microfluidic platform to study age-dependent protein abundance and localization changes in Saccharomyces cerevisiae
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion