RNA-binding proteins mediate the maturation of chromatin topology during differentiation

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• dc.contributor.author Dehingia, Bondita
• dc.contributor.author Severino, Jacqueline, 1990-
• dc.contributor.author Pękowska, Aleksandra
• dc.date.accessioned 2025-10-14T05:36:31Z
• dc.date.available 2025-10-14T05:36:31Z
• dc.date.issued 2025
• dc.description.abstract Topologically associating domains (TADs) and chromatin architectural loops impact promoter-enhancer interactions, with CCCTC-binding factor (CTCF) defining TAD borders and loop anchors. TAD boundaries and loops progressively strengthen upon embryonic stem (ES) cell differentiation, underscoring the importance of chromatin topology in ontogeny. However, the mechanisms driving this process remain unclear. Here we show a widespread increase in CTCF-RNA-binding protein (RBP) interactions upon ES to neural stem (NS) cell differentiation. While dispensable in ES cells, RBPs reinforce CTCF-anchored chromatin topology in NS cells. We identify Pantr1, a non-coding RNA, as a key facilitator of CTCF-RBP interactions, promoting chromatin maturation. Using acute CTCF degradation, we find that, through its insulator function, CTCF helps maintain neuronal gene silencing in NS cells by acting as a barrier to untimely gene activation during development. Altogether, we reveal a fundamental mechanism driving developmentally linked chromatin structural consolidation and the contribution of this process to the control of gene expression in differentiation.
• dc.format.mimetype application/pdf
• dc.identifier.citation Dehingia B, Milewska-Puchała M, Janowski M, Rafiee MR, Abbas M, Piotrowska A, et al. RNA-binding proteins mediate the maturation of chromatin topology during differentiation. Nat Cell Biol. 2025 Sep;27(9):1510-25. DOI: 10.1038/s41556-025-01735-5
• dc.identifier.doi http://dx.doi.org/10.1038/s41556-025-01735-5
• dc.identifier.issn 1465-7392
• dc.identifier.uri http://hdl.handle.net/10230/71495
• dc.language.iso eng
• dc.publisher Nature Research
• dc.relation.ispartof Nature cell biology. 2025 Sep;27(9):1510-25
• dc.rights © The Author(s) 2025. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Differentiation
• dc.subject.keyword Nuclear organization
• dc.subject.keyword Stem-cell differentiation
• dc.title RNA-binding proteins mediate the maturation of chromatin topology during differentiation
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion