Characterization of ecologically diverse viruses infecting co-occurring strains of cosmopolitan hyperhalophilic bacteroidetes

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• dc.contributor.author Villamor, Judith
• dc.contributor.author Ramos-Barbero, María Dolores
• dc.contributor.author González-Torres, Pedro
• dc.contributor.author Gabaldón Estevan, Juan Antonio, 1973-
• dc.contributor.author Rosselló-Mora, Ramón
• dc.contributor.author Meseguer, Inmaculada
• dc.contributor.author Martínez García, Manuel
• dc.contributor.author Santos, Fernando
• dc.contributor.author Antón, Josefa
• dc.date.accessioned 2019-10-31T08:41:29Z
• dc.date.available 2019-10-31T08:41:29Z
• dc.date.issued 2018
• dc.description.abstract Hypersaline environments close to saturation harbor the highest density of virus-like particles reported for aquatic systems as well as low microbial diversity. Thus, they offer unique settings for studying virus-host interactions in nature. However, no viruses have been isolated so far infecting the two most abundant inhabitants of these systems (that is, the euryarchaeon Haloquadratum walsbyi and the bacteroidetes Salinibacter ruber). Here, using three different co-occurring strains, we have isolated eight viruses infecting the ubiquitous S. ruber that constitute three new different genera (named as 'Holosalinivirus', 'Kryptosalinivirus' and 'Kairosalinivirus') according to their genomic traits, different host range, virus-host interaction capabilities and abundances in natural systems worldwide. Furthermore, to get a more complete and comprehensive view of S. ruber virus assemblages in nature, a microcosm experiment was set with a mixture of S. ruber strains challenged with a brine virus concentrate, and changes of viral populations were monitored by viral metagenomics. Only viruses closely related to kairosalinivirus (strictly lytic and wide host range) were enriched, despite their low initial abundance in the natural sample. Metagenomic analyses of the mesocosms allowed the complete recovery of kairosalinivirus genomes using an ad hoc assembly strategy as common viral metagenomic assembly tools failed despite their abundance, which underlines the limitations of current approaches. The increase of this type of viruses was accompanied by an increase in the diversity of the group, as shown by contig recruitment. These results are consistent with a scenario in which host range, not only virus and host abundances, is a key factor in determining virus fate in nature.
• dc.description.sponsorship This research was supported by the Spanish Ministry of Economy projects CLG2015_66686-C3-1 (to JA) and CLG2015_66686-C3-3 (to RRM), which were also supported with European Regional Development Fund (FEDER) funds.
• dc.format.mimetype application/pdf
• dc.identifier.citation Villamor J, Ramos-Barbero MD, González-Torres P, Gabaldón T, Rosselló-Móra R, Meseguer I, Martínez-García M, Santos F, Antón J. Characterization of ecologically diverse viruses infecting co-occurring strains of cosmopolitan hyperhalophilic bacteroidetes. ISME J. 2018; 12(2): 424-437. DOI 10.1038/ismej.2017.175
• dc.identifier.doi http://dx.doi.org/10.1038/ismej.2017.175
• dc.identifier.issn 1751-7362
• dc.identifier.uri http://hdl.handle.net/10230/42571
• dc.language.iso eng
• dc.publisher Nature Research
• dc.relation.ispartof ISME J. 2018; 12(2): 424-437
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/CLG2015_66686-C3-1
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/CLG2015_66686-C3-3
• dc.rights © The Author(s) 2018. This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/4.0/
• dc.subject.keyword Biodiversity
• dc.subject.keyword Metagenomics
• dc.subject.keyword Virus–host interactions
• dc.title Characterization of ecologically diverse viruses infecting co-occurring strains of cosmopolitan hyperhalophilic bacteroidetes
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion