Transition of human γ-tubulin ring complex into a closed conformation during microtubule nucleation

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• dc.contributor.author Brito, Cláudia
• dc.contributor.author Serna, Marina
• dc.contributor.author Guerra, Pablo
• dc.contributor.author Llorca, Óscar
• dc.contributor.author Surrey, Thomas
• dc.date.accessioned 2024-02-14T16:52:33Z
• dc.date.available 2024-02-14T16:52:33Z
• dc.date.issued 2024
• dc.description.abstract Microtubules are essential for intracellular organization and chromosome segregation. They are nucleated by the γ-tubulin ring complex (γTuRC). However, isolated vertebrate γTuRC adopts an open conformation that deviates from the microtubule structure, raising the question of the nucleation mechanism. Here we determine cryo-electron microscopy structures of human γTuRC bound to a nascent microtubule. Structural changes of the complex into a closed conformation ensure that γTuRC templates the 13-protofilament microtubules that exist in human cells. Closure is mediated by a latch that interacts with incorporating tubulin, making it part of the closing mechanism. Further rearrangements involve all γ-tubulin ring complex subunits and the removal of the actin-containing luminal bridge. Our proposed mechanism of microtubule nucleation by human γTuRC relies on large-scale structural changes that are likely the target of regulation in cells.
• dc.format.mimetype application/pdf
• dc.identifier.citation Brito C, Serna M, Guerra P, Llorca O, Surrey T. Transition of human γ-tubulin ring complex into a closed conformation during microtubule nucleation. Science. 2024 Feb 23;383(6685):870-6. DOI: 10.1126/science.adk6160
• dc.identifier.doi http://dx.doi.org/10.1126/science.adk6160
• dc.identifier.issn 0036-8075
• dc.identifier.uri http://hdl.handle.net/10230/59109
• dc.language.iso eng
• dc.publisher American Association for the Advancement of Science (AAAS)
• dc.relation.ispartof Science. 2024 Feb 23;383(6685):870-6
• dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/951430
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/CEX2020-001049-S
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PID2019-108415GB-I00
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PID2020-114429RB-I00
• dc.rights This is the author’s version of the work. It is posted here by permission of the AAAS for personal use, not for redistribution. The definitive version was published in Science, DOI: 10.1126/science.adk6160.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.subject.other Microtúbuls
• dc.subject.other Tubulines
• dc.title Transition of human γ-tubulin ring complex into a closed conformation during microtubule nucleation
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/acceptedVersion