Recent origin of the methacrylate redox system in geobacter sulfurreducens AM-1 through horizontal gene transfer

Full item page Simple item page

• dc.contributor.author Arkhipova, Oksana V.ca
• dc.contributor.author Meer, Margarita V., 1986-ca
• dc.contributor.author Mikoulinskaia, Galina V.ca
• dc.contributor.author Zakharova, Marina V.ca
• dc.contributor.author Galushko, Alexander S.ca
• dc.contributor.author Akimenko, Vasilii K.ca
• dc.contributor.author Kondrashov, Fyodor A., 1979-ca
• dc.date.accessioned 2015-11-19T14:01:05Z
• dc.date.available 2015-11-19T14:01:05Z
• dc.date.issued 2015
• dc.description.abstract The origin and evolution of novel biochemical functions remains one of the key questions in molecular evolution. We study recently emerged methacrylate reductase function that is thought to have emerged in the last century and reported in Geobacter sulfurreducens strain AM-1. We report the sequence and study the evolution of the operon coding for the flavin-containing methacrylate reductase (Mrd) and tetraheme cytochrome с (Mcc) in the genome of G. sulfurreducens AM-1. Different types of signal peptides in functionally interlinked proteins Mrd and Mcc suggest a possible complex mechanism of biogenesis for chromoproteids of the methacrylate redox system. The homologs of the Mrd and Mcc sequence found in δ-Proteobacteria and Deferribacteres are also organized into an operon and their phylogenetic distribution suggested that these two genes tend to be horizontally transferred together. Specifically, the mrd and mcc genes from G. sulfurreducens AM-1 are not monophyletic with any of the homologs found in other Geobacter genomes. The acquisition of methacrylate reductase function by G. sulfurreducens AM-1 appears linked to a horizontal gene transfer event. However, the new function of the products of mrd and mcc may have evolved either prior or subsequent to their acquisition by G. sulfurreducens AM-1.en
• dc.description.sponsorship The work has been supported by a grant of the HHMI International Early Career Scientist Program (55007424), the Spanish Ministry of Economy and Competitiveness (EUI-EURYIP-2011-4320) as part of the EMBO YIP program, two grants from the Spanish Ministry of Economy and Competitiveness, "Centro de Excelencia Severo Ochoa 2013–2017 (Sev-2012-0208)" and (BFU2012-31329), the European Union and the European Research Council under grant agreement/n335980_EinME.en
• dc.format.mimetype application/pdfca
• dc.identifier.citation Arkhipova OV, Meer MV, Mikoulinskaia GV, Zakharova MV, Galushko AS, Akimenko VK et al. Recent origin of the methacrylate redox system in geobacter sulfurreducens AM-1 through horizontal gene transfer. PLoS ONE. 2015;10(5):e0125888. DOI: 10.1371/journal.pone.0125888ca
• dc.identifier.doi http://dx.doi.org/10.1371/journal.pone.0125888
• dc.identifier.issn 1932-6203
• dc.identifier.uri http://hdl.handle.net/10230/25156
• dc.language.iso engca
• dc.publisher Public Library of Science (PLoS)ca
• dc.relation.ispartof PLoS ONE. 2015;10(5):e0125888
• dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/335980
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/SEV2012-0208
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2012-31329
• dc.rights © 2015 Arkhipova et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
• dc.rights.accessRights info:eu-repo/semantics/openAccessca
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/ca
• dc.subject.other Evolució molecular
• dc.title Recent origin of the methacrylate redox system in geobacter sulfurreducens AM-1 through horizontal gene transferen
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/publishedVersionca