Cell-Free DNA as a biomarker at diagnosis and follow-up in 256 B and T-Cell lymphomas

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• dc.contributor.author Díez-Feijóo, Ramón
• dc.contributor.author Andrade-Campos, Marcio
• dc.contributor.author Gibert Fernandez, Joan, 1988-
• dc.contributor.author Sánchez González, Blanca
• dc.contributor.author Fernández-Ibarrondo, Lierni
• dc.contributor.author Fernández Rodríguez, M. Concepción
• dc.contributor.author García Gisbert, Nieves, 1994-
• dc.contributor.author Camacho Díaz, Laura
• dc.contributor.author Lafuente, Marta
• dc.contributor.author Vázquez, Ivonne
• dc.contributor.author Colomo Saperas, Luis Alberto
• dc.contributor.author Salar Silvestre, Antonio
• dc.contributor.author Bellosillo Paricio, Beatriz
• dc.date.accessioned 2024-10-08T06:26:18Z
• dc.date.available 2024-10-08T06:26:18Z
• dc.date.issued 2024
• dc.description.abstract Background: Cell-free DNA (cfDNA) analysis has become a promising tool for the diagnosis, prognosis, and monitoring of lymphoma cases. Until now, research in this area has mainly focused on aggressive lymphomas, with scanty information from other lymphoma subtypes. Methods: We selected 256 patients diagnosed with lymphomas, including a large variety of B-cell and T-cell non-Hodgkin and Hodgkin lymphomas, and quantified cfDNA from plasma at the time of diagnosis. We further selected 49 large B-cell lymphomas (LBCL) and analyzed cfDNA levels at diagnosis (pre-therapy) and after therapy. In addition, we performed NGS on cfDNA and tissue in this cohort of LBCL. Results: Lymphoma patients showed a statistically significant higher cfDNA concentration than healthy controls (mean 53.0 ng/mL vs. 5.6 ng/mL, p < 0.001). The cfDNA concentration was correlated with lymphoma subtype, lactate dehydrogenase, the International Prognostic Index (IPI) score, Ann Arbor (AA), and B-symptoms. In 49 LBCL cases, the cfDNA concentration decreased after therapy in cases who achieved complete response (CR) and increased in non-responders. The median cfDNA at diagnosis of patients who achieved CR and later relapsed was higher (81.5 ng/mL) compared with levels of those who did not (38.6 ng/mL). A concordance of 84% was observed between NGS results in tumor and cfDNA samples. Higher VAF in cfDNA is correlated with advanced stage and bulky disease. Conclusions: cfDNA analysis can be easily performed in almost all lymphoma cases. The cfDNA concentration correlated with the characteristics of the aggressiveness of the lymphomas and, in LBCL, with the response achieved after therapy. These results support the utility of cfDNA analysis as a complementary tool in the management of lymphoma patients.
• dc.format.mimetype application/pdf
• dc.identifier.citation Diez-Feijóo R, Andrade-Campos M, Gibert J, Sánchez-González B, Fernández-Ibarrondo L, Fernández-Rodríguez C, et al. Cell-Free DNA as a biomarker at diagnosis and follow-up in 256 B and T-Cell lymphomas. Cancers (Basel). 2024 Jan 11;16(2):321. DOI: 10.3390/cancers16020321
• dc.identifier.doi http://dx.doi.org/10.3390/cancers16020321
• dc.identifier.issn 2072-6694
• dc.identifier.uri http://hdl.handle.net/10230/61340
• dc.language.iso eng
• dc.publisher MDPI
• dc.relation.ispartof Cancers (Basel). 2024 Jan 11;16(2):321
• dc.rights © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword cfDNA
• dc.subject.keyword Liquid biopsy
• dc.subject.keyword Lymphoma
• dc.subject.keyword Monitoring
• dc.title Cell-Free DNA as a biomarker at diagnosis and follow-up in 256 B and T-Cell lymphomas
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion