In vitro bioassays for monitoring drinking water quality of tap water, domestic filtration and bottled water

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• dc.contributor.author Escher, Beate I.
• dc.contributor.author Blanco, Jordi
• dc.contributor.author Caixach, Josep
• dc.contributor.author Cserbik, Dora
• dc.contributor.author Farré, Maria José
• dc.contributor.author Flores, Cintia
• dc.contributor.author König, Maria
• dc.contributor.author Lee, Jungeun
• dc.contributor.author Nyffeler, Johanna
• dc.contributor.author Planas, Carles
• dc.contributor.author Redondo-Hasselerharm, Paula E.
• dc.contributor.author Rovira, Joaquim
• dc.contributor.author Sanchís, Josep
• dc.contributor.author Schuhmacher, Marta
• dc.contributor.author Villanueva, Cristina M.
• dc.date.accessioned 2023-08-31T13:12:46Z
• dc.date.available 2023-08-31T13:12:46Z
• dc.date.issued 2024
• dc.description.abstract Background: Location-specific patterns of regulated and non-regulated disinfection byproducts (DBPs) were detected in tap water samples of the Barcelona Metropolitan Area. However, it remains unclear if the detected DBPs together with undetected DPBs and organic micropollutants can lead to mixture effects in drinking water. Objective: To evaluate the neurotoxicity, oxidative stress response and cytotoxicity of 42 tap water samples, 6 treated with activated carbon filters, 5 with reverse osmosis and 9 bottled waters. To compare the measured effects of the extracts with the mixture effects predicted from the detected concentrations and the relative effect potencies of the detected DBPs using the mixture model of concentration addition. Methods: Mixtures of organic chemicals in water samples were enriched by solid phase extraction and tested for cytotoxicity and neurite outgrowth inhibition in the neuronal cell line SH-SY5Y and for cytotoxicity and oxidative stress response in the AREc32 assay. Results: Unenriched water did not trigger neurotoxicity or cytotoxicity. After up to 500-fold enrichment, few extracts showed cytotoxicity. Disinfected water showed low neurotoxicity at 20- to 300-fold enrichment and oxidative stress response at 8- to 140-fold enrichment. Non-regulated non-volatile DBPs, particularly (brominated) haloacetonitriles dominated the predicted mixture effects of the detected chemicals and predicted effects agreed with the measured effects. By hierarchical clustering we identified strong geographical patterns in the types of DPBs and their association with effects. Activated carbon filters did not show a consistent reduction of effects but domestic reverse osmosis filters decreased the effect to that of bottled water. Impact statement: Bioassays are an important complement to chemical analysis of disinfection by-products (DBPs) in drinking water. Comparison of the measured oxidative stress response and mixture effects predicted from the detected chemicals and their relative effect potencies allowed the identification of the forcing agents for the mixture effects, which differed by location but were mainly non-regulated DBPs. This study demonstrates the relevance of non-regulated DBPs from a toxicological perspective. In vitro bioassays, in particular reporter gene assays for oxidative stress response that integrate different reactive toxicity pathways including genotoxicity, may therefore serve as sum parameters for drinking water quality assessment.
• dc.format.mimetype application/pdf
• dc.identifier.citation Escher BI, Blanco J, Caixach J, Cserbik D, Farré MJ, Flores C, et al. In vitro bioassays for monitoring drinking water quality of tap water, domestic filtration and bottled water. J Expo Sci Environ Epidemiol. 2024 Jan;34(1):126-35. DOI: 10.1038/s41370-023-00566-6
• dc.identifier.doi http://dx.doi.org/10.1038/s41370-023-00566-6
• dc.identifier.issn 1559-0631
• dc.identifier.uri http://hdl.handle.net/10230/57788
• dc.language.iso eng
• dc.publisher Nature Research
• dc.relation.ispartof J Expo Sci Environ Epidemiol. 2024 Jan;34(1):126-35
• dc.rights © The Author(s) 2023. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Bioassay
• dc.subject.keyword Disinfection by-products
• dc.subject.keyword Neurotoxicity
• dc.subject.keyword Oxidative stress
• dc.subject.keyword Water quality
• dc.title In vitro bioassays for monitoring drinking water quality of tap water, domestic filtration and bottled water
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion