Characterisation of novel microRNAs in the Black flying fox (Pteropus alecto) by deep sequencing

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• dc.contributor.author Cowled, Christopherca
• dc.contributor.author Stewart, Cameron Rca
• dc.contributor.author Likic, Vladimir Aca
• dc.contributor.author Friedländer, Marc R.ca
• dc.contributor.author Tachedjian, Maryca
• dc.contributor.author Jenkins, Kristie Aca
• dc.contributor.author Tizard, Mark Lca
• dc.contributor.author Cottee, Paulineca
• dc.contributor.author Marsh, Glenn Aca
• dc.contributor.author Zhou, Pengca
• dc.contributor.author Baker, Michelle Lca
• dc.contributor.author Bean, Andrew Gca
• dc.contributor.author Wang, Lin-faca
• dc.date.accessioned 2015-03-24T12:06:54Z
• dc.date.available 2015-03-24T12:06:54Z
• dc.date.issued 2014ca
• dc.description.abstract Background: Bats are a major source of new and emerging viral diseases. Despite the fact that bats carry and shed highly pathogenic viruses including Ebola, Nipah and SARS, they rarely display clinical symptoms of infection. Host factors influencing viral replication are poorly understood in bats and are likely to include both pre- and post-transcriptional regulatory mechanisms. MicroRNAs are a major mechanism of post-transcriptional gene regulation, however very little is known about them in bats. Results: This study describes 399 microRNAs identified by deep sequencing of small RNA isolated from tissues of the Black flying fox, Pteropus alecto, a confirmed natural reservoir of the human pathogens Hendra virus and Australian bat lyssavirus. Of the microRNAs identified, more than 100 are unique amongst vertebrates, including a subset containing mutations in critical seed regions. Clusters of rapidly-evolving microRNAs were identified, as well as microRNAs predicted to target genes involved in antiviral immunity, the DNA damage response, apoptosis and autophagy. Closer inspection of the predicted targets for several highly supported novel miRNA candidates suggests putative roles in host-virus interaction. Conclusions: MicroRNAs are likely to play major roles in regulating virus-host interaction in bats, via dampening of inflammatory responses (limiting the effects of immunopathology), and directly limiting the extent of viral replication, either through restricting the availability of essential factors or by controlling apoptosis. Characterisation of the bat microRNA repertoire is an essential step towards understanding transcriptional regulation during viral infection, and will assist in the identification of mechanisms that enable bats to act as natural virus reservoirs. This in turn will facilitate the development of antiviral strategies for use in humans and other species.en
• dc.description.sponsorship M.R.F. is supported by EMBO Long-Term fellowship ALTF 225–2011en
• dc.format.mimetype application/pdfca
• dc.identifier.citation Cowled C, Stewart CR, Likic VA, Friedländer MR, Tachedjian M, Jenkins KA, Tizard ML, Cottee P, Marsh GA, Zhou P, Baker ML, Bean AG, Wang LF. Characterisation of novel microRNAs in the Black flying fox (Pteropus alecto) by deep sequencing. BMC Genomics. 2014; 15: 682. DOI 10.1186/1471-2164-15-682ca
• dc.identifier.doi http://dx.doi.org/10.1186/1471-2164-15-682
• dc.identifier.issn 1471-2164ca
• dc.identifier.uri http://hdl.handle.net/10230/23258
• dc.language.iso engca
• dc.publisher BioMed Centralca
• dc.relation.ispartof BMC Genomics. 2014; 15: 682
• dc.rights © 2014 Cowled et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.ca
• dc.rights.accessRights info:eu-repo/semantics/openAccessca
• dc.rights.uri http://creativecommons.org/licenses/by/2.0
• dc.subject.keyword Batsen
• dc.subject.keyword Chiropteraen
• dc.subject.keyword Pteropus alectoen
• dc.subject.keyword MicroRNAen
• dc.subject.keyword Non-coding RNAen
• dc.subject.keyword Transcriptomeen
• dc.subject.other RNA no missatgersca
• dc.subject.other Regulació genèticaca
• dc.subject.other Infeccióca
• dc.title Characterisation of novel microRNAs in the Black flying fox (Pteropus alecto) by deep sequencingen
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/publishedVersionca