Experimental setting for applying mechanical stimuli to study the endothelial response of ex vivo vessels under realistic pathophysiological environments

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• dc.contributor.author Osuna, Ana
• dc.contributor.author Ulldemolins, Anna
• dc.contributor.author Sanz-Fraile, Hector
• dc.contributor.author Otero, Jorge
• dc.contributor.author Farré López, Núria
• dc.contributor.author Farré, Ramon
• dc.contributor.author Almendros, Isaac
• dc.date.accessioned 2022-06-28T07:00:33Z
• dc.date.available 2022-06-28T07:00:33Z
• dc.date.issued 2021
• dc.description.abstract This paper describes the design, construction and testing of an experimental setting, making it possible to study the endothelium under different pathophysiological conditions. This novel experimental approach allows the application of the following stimuli to an ex vivo vessel in a physiological bath: (a) a realistic intravascular pressure waveform defined by the user; (b) shear stress in the endothelial layer since, in addition to the pressure waveform, the flow through the vessel can be independently controlled by the user; (c) conditions of hypo/hyperoxia and hypo/hypercapnia in an intravascular circulating medium. These stimuli can be applied alone or in different combinations to study possible synergistic or antagonistic effects. The setting performance is illustrated by a proof of concept in an ex vivo rabbit aorta. The experimental setting is easy to build by using very low-cost materials widely available. Online Supplement files provide all the technical information (e.g., circuits, codes, 3D printer drivers) following an open-source hardware approach for free replication.
• dc.description.sponsorship This work was supported in part by the Spanish Ministry of Science, Innovation and Universities (SAF2017-85574-R, PGC2018-097323-A-I00, PID2019-108958RB-I00).
• dc.format.mimetype application/pdf
• dc.identifier.citation Osuna A, Ulldemolins A, Sanz-Fraile H, Otero J, Farré N, Farré R, et al. Experimental setting for applying mechanical stimuli to study the endothelial response of ex vivo vessels under realistic pathophysiological environments. Life (Basel). 2021 Jul 8; 11(7): 671. DOI: 10.3390/life11070671
• dc.identifier.doi http://dx.doi.org/10.3390/life11070671
• dc.identifier.issn 2075-1729
• dc.identifier.uri http://hdl.handle.net/10230/53609
• dc.language.iso eng
• dc.publisher MDPI
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/SAF2017-85574-R
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PGC2018-097323-A-I00
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PID2019-108958RB-I00
• dc.rights Copyright © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Bioreactor
• dc.subject.keyword Experimental physiology
• dc.subject.keyword Hypoxia
• dc.subject.keyword Mechanical stimuli
• dc.subject.keyword Open-source hardware
• dc.subject.keyword Vessel mechanics
• dc.title Experimental setting for applying mechanical stimuli to study the endothelial response of ex vivo vessels under realistic pathophysiological environments
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion