The Conserved Non-Coding Sequence 2 (CNS2) enhances CD69 transcription through cooperation between the transcription factors Oct1 and RUNX1

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• dc.contributor.author Fontela, Miguel G.
• dc.contributor.author Notario, Laura
• dc.contributor.author Alari-Pahissa, Elisenda
• dc.contributor.author Lorente, Elena
• dc.contributor.author Lauzurica, Pilar
• dc.date.accessioned 2020-06-11T07:16:32Z
• dc.date.available 2020-06-11T07:16:32Z
• dc.date.issued 2019
• dc.description.abstract The immune regulatory receptor CD69 is expressed upon activation in all types of leukocytes and is strongly regulated at the transcriptional level. We previously described that, in addition to the CD69 promoter, there are four conserved noncoding regions (CNS1-4) upstream of the CD69 promoter. Furthermore, we proposed that CNS2 is the main enhancer of CD69 transcription. In the present study, we mapped the transcription factor (TF) binding sites (TFBS) from ChIP-seq databases within CNS2. Through luciferase reporter assays, we defined a ~60 bp sequence that acts as the minimum enhancer core of mouse CNS2, which includes the Oct1 TFBS. This enhancer core establishes cooperative interactions with the 3' and 5' flanking regions, which contain RUNX1 BS. In agreement with the luciferase reporter data, the inhibition of RUNX1 and Oct1 TF expression by siRNA suggests that they synergistically enhance endogenous CD69 gene transcription. In summary, we describe an enhancer core containing RUNX1 and Oct1 BS that is important for the activity of the most potent CD69 gene transcription enhancer.
• dc.format.mimetype application/pdf
• dc.identifier.citation Fontela MG, Notario L, Alari-Pahissa E, Lorente E, Lauzurica P. The Conserved Non-Coding Sequence 2 (CNS2) enhances CD69 transcription through cooperation between the transcription factors Oct1 and RUNX1. Genes (Basel). 2019; 10(9):651. DOI: 10.3390/genes10090651
• dc.identifier.doi http://dx.doi.org/10.3390/genes10090651
• dc.identifier.issn 2073-4425
• dc.identifier.uri http://hdl.handle.net/10230/44959
• dc.language.iso eng
• dc.publisher MDPI
• dc.relation.ispartof Genes (Basel). 2019; 10(9):651
• dc.rights © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword CD69
• dc.subject.keyword Enhancer
• dc.subject.keyword Noncoding regions
• dc.subject.keyword Transcription factor
• dc.subject.keyword Transcriptional regulation
• dc.title The Conserved Non-Coding Sequence 2 (CNS2) enhances CD69 transcription through cooperation between the transcription factors Oct1 and RUNX1
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion