IKKα is required in the intestinal epithelial cells for tumour stemness

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• dc.contributor.author Colomer Montañà, Carlota, 1990-ca
• dc.contributor.author Margalef González, Pol, 1985-ca
• dc.contributor.author González, Jessicaca
• dc.contributor.author Vert, Annaca
• dc.contributor.author Bigas Salvans, Annaca
• dc.contributor.author Espinosa Blay, Lluísca
• dc.date.accessioned 2018-10-19T07:17:21Z
• dc.date.issued 2018
• dc.description.abstract BACKGROUND: Colorectal cancer is a common cause of death in developed countries. Progression from adenoma to invasive carcinoma requires accumulation of mutations starting with the Adenomatous Polyposis Coli (Apc) gene. NF-κB signalling is a key element in cancer, mainly related to the activity of IKKβ. IKKα kinase also participates in this process by mechanisms that are primarily unknown. METHODS: We generated a compound mouse model with mutation in Apc and lacking intestinal epithelial IKKα, produced intestinal organoids and tumour spheroids with different genetic backgrounds, and performed immunohistochemistry and RNA-seq analysis. RESULTS: Deficiency of IKKα prevents adenoma formation, with adenomas lacking IKKα showing reduced proliferation. In contrast, IKKα status did not affect normal intestinal function. The same divergent phenotype was found in the organoid-spheroid model. We also found that epithelial IKKα controls stemness, proliferation and apoptosis-related expression. CONCLUSIONS: IKKα is a potential therapeutic target for Apc mutant colorectal cancer patients
• dc.format.mimetype application/pdf
• dc.identifier.citation Colomer C, Margalef P, Gonzalez J, Vert A, Bigas A, Espinosa L. IKKα is required in the intestinal epithelial cells for tumour stemness. Br J Cancer. 2018 Mar 20;118(6):839-846. DOI: 10.1038/bjc.2017.459
• dc.identifier.doi http://dx.doi.org/ 10.1038/bjc.2017.459
• dc.identifier.issn 0007-0920
• dc.identifier.uri http://hdl.handle.net/10230/35623
• dc.language.iso eng
• dc.publisher Nature Publishing Groupca
• dc.relation.ispartof British Journal of Cancer. 2018 Mar 20;118(6):839-46
• dc.rights © Nature Publishing Group. http://dx.doi.org/ 10.1038/bjc.2017.459
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.subject.other Còlon -- Càncer -- Aspectes genètics
• dc.title IKKα is required in the intestinal epithelial cells for tumour stemnessca
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/acceptedVersion