Stably expressed APOBEC3H forms a barrier for cross-species transmission of simian immunodeficiency virus of chimpanzee to humans

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• dc.contributor.author Zhang, Zelica
• dc.contributor.author Gu, Qinyongca
• dc.contributor.author de Manuel, Marcca
• dc.contributor.author Bravo, Ignacio G.ca
• dc.contributor.author Marquès i Bonet, Tomàs, 1975-ca
• dc.contributor.author Häussinger, Diéterca
• dc.contributor.author Münk, Carstenca
• dc.date.accessioned 2018-03-22T08:44:26Z
• dc.date.available 2018-03-22T08:44:26Z
• dc.date.issued 2017
• dc.description.abstract APOBEC3s (A3s) are potent restriction factors of human immunodeficiency virus type 1/simian immunodeficiency viruses (HIV-1/SIV), and can repress cross-species transmissions of lentiviruses. HIV-1 originated from a zoonotic infection of SIV of chimpanzee (SIVcpz) to humans. However, the impact of human A3s on the replication of SIVcpz remains unclear. By using novel SIVcpz reporter viruses, we identified that human APOBEC3B (A3B) and APOBEC3H (A3H) haplotype II strongly reduced the infectivity of SIVcpz, because both of them are resistant to SIVcpz Vifs. We further demonstrated that human A3H inhibited SIVcpz by deaminase dependent as well independent mechanisms. In addition, other stably expressed human A3H haplotypes and splice variants showed strong antiviral activity against SIVcpz. Moreover, most SIV and HIV lineage Vif proteins could degrade chimpanzee A3H, but no Vifs from SIVcpz and SIV of gorilla (SIVgor) lineages antagonized human A3H haplotype II. Expression of human A3H hapII in human T cells efficiently blocked the spreading replication of SIVcpz. The spreading replication of SIVcpz was also restricted by stable A3H in human PBMCs. Thus, we speculate that stably expressed human A3H protects humans against the cross-species transmission of SIVcpz and that SIVcpz spillover to humans may have started in individuals that harbor haplotypes of unstable A3H proteins.
• dc.format.mimetype application/pdf
• dc.identifier.citation Zhang Z, Gu Q, de Manuel Montero M, Bravo IG, Marques-Bonet T, Häussinger D et al. Stably expressed APOBEC3H forms a barrier for cross-species transmission of simian immunodeficiency virus of chimpanzee to humans. PLoS Pathog. 2017 Dec 21;13(12):e1006746. DOI: 10.1371/journal.ppat.1006746
• dc.identifier.doi http://dx.doi.org/10.1371/journal.ppat.1006746
• dc.identifier.issn 1553-7366
• dc.identifier.uri http://hdl.handle.net/10230/34237
• dc.language.iso eng
• dc.publisher Public Library of Science (PLoS)ca
• dc.relation.ispartof PLOS Pathogens. 2017 Dec 21;13(12):e1006746
• dc.rights © 2017 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Aminohydrolases
• dc.subject.keyword 293T cells
• dc.subject.keyword Plasmid construction
• dc.subject.keyword HIV-1
• dc.subject.keyword SIV
• dc.title Stably expressed APOBEC3H forms a barrier for cross-species transmission of simian immunodeficiency virus of chimpanzee to humansca
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion