Clustering of Drosophila housekeeping promoters facilitates their expression
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- dc.contributor.author Corrales Berjano, Marc, 1984-ca
- dc.contributor.author Rosado, Aránzazuca
- dc.contributor.author Cortini, Ruggeroca
- dc.contributor.author van Arensbergen, Jorisca
- dc.contributor.author van Steensel, Basca
- dc.contributor.author Filion, Guillaumeca
- dc.date.accessioned 2018-06-14T07:43:28Z
- dc.date.available 2018-06-14T07:43:28Z
- dc.date.issued 2017
- dc.description.abstract Housekeeping genes of animal genomes cluster in the same chromosomal regions. It has long been suggested that this organization contributes to their steady expression across all the tissues of the organism. Here, we show that the activity of Drosophila housekeeping gene promoters depends on the expression of their neighbors. By measuring the expression of ∼85,000 reporters integrated in Kc167 cells, we identified the best predictors of expression as chromosomal contacts with the promoters and terminators of active genes. Surprisingly, the chromatin composition at the insertion site and the contacts with enhancers were less informative. These results are substantiated by the existence of genomic "paradoxical" domains, rich in euchromatic features and enhancers, but where the reporters are expressed at low level, concomitant with a deficit of interactions with promoters and terminators. This indicates that the proper function of housekeeping genes relies not on contacts with long distance enhancers but on spatial clustering. Overall, our results suggest that spatial proximity between genes increases their expression and that the linear architecture of the Drosophila genome contributes to this effect.
- dc.description.sponsorship This work was supported by the Spanish Ministry of Economy and Competitiveness, “Centro de Excelencia Severo Ochoa 2013-2017,” SEV-2012-0208, Plan Nacional BFU2012-37168, NWO-ALW VICI, ERC Advanced Grant 293662 (B.v.S.), ERC Synergy Grant 609989 (G.J.F.), and the People Programme (Marie Curie Actions) of the European Union's Seventh Framework Programme (FP7/2007-2013) under REA grant agreement no. 608959 (R.C.).
- dc.format.mimetype application/pdf
- dc.identifier.citation Corrales M, Rosado A, Cortini R, van Arensbergen J, van Steensel B, Filion GJ. Clustering of Drosophila housekeeping promoters facilitates their expression. Genome Res. 2017 Jul;27(7):1153-61. DOI: 10.1101/gr.211433.116
- dc.identifier.doi http://dx.doi.org/10.1101/gr.211433.116
- dc.identifier.issn 1088-9051
- dc.identifier.uri http://hdl.handle.net/10230/34898
- dc.language.iso eng
- dc.publisher Cold Spring Harbor Laboratory Press (CSHL Press)ca
- dc.relation.ispartof Genome Research. 2017 Jul;27(7):1153-61
- dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/293662
- dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2012-37168
- dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/609989
- dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/608959
- dc.rights © 2017 Corrales et al. This article, published in Genome Research, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by-nc/4.0/
- dc.subject.keyword Drosophila melanogaster
- dc.subject.keyword Gene expression regulation
- dc.subject.keyword Multigene family
- dc.title Clustering of Drosophila housekeeping promoters facilitates their expressionca
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion