Engineered marine biofilms for ocean environment monitoring

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• dc.contributor.author Nevot, Guillermo
• dc.contributor.author Pol Cros, Maria
• dc.contributor.author Toloza, Lorena
• dc.contributor.author Campamà-Sanz, Nil
• dc.contributor.author Artigues-Lleixà, Maria
• dc.contributor.author Aguilera, Laura
• dc.contributor.author Güell Cargol, Marc, 1982-
• dc.date.accessioned 2025-07-25T06:18:17Z
• dc.date.available 2025-07-25T06:18:17Z
• dc.date.issued 2025
• dc.description.abstract Marine bacteria offer a promising alternative for developing Engineered Living Materials (ELMs) tailored to marine applications. We engineered Dinoroseobacter shibae to increase its surface-associated growth and develop biosensors for ocean environment monitoring. By fusing the endogenous extracellular matrix amyloidogenic protein CsgA with mussel foot proteins, we significantly increased D. shibae biofilm formation. Additionally, D. shibae was engineered to express the tyrosinase enzyme to further enhance microbial attachment through post-translational modifications of tyrosine residues. By exploiting D. shibae's natural genetic resources, two environmental biosensors were created to detect temperature and oxygen. These biosensors were coupled with a CRISPR-based recording system to store transient gene expression in stable DNA arrays, enabling long-term environmental monitoring. These engineered strains highlight D. shibae's potential in advancing marine microbiome engineering for innovative biofilm applications, including the development of natural, self-renewing biological adhesives, environmental sensors, and "sentinel" cells equipped with CRISPR-recording technology to capture and store environmental signals.
• dc.format.mimetype application/pdf
• dc.identifier.citation Nevot G, Pol Cros M, Toloza L, Campamà-Sanz N, Artigues-Lleixà M, Aguilera L, et al. Engineered marine biofilms for ocean environment monitoring. ACS Synth Biol. 2025 Jul 18;14(7):2797-809. DOI: 10.1021/acssynbio.5c00192
• dc.identifier.doi http://dx.doi.org/10.1021/acssynbio.5c00192
• dc.identifier.issn 2161-5063
• dc.identifier.uri http://hdl.handle.net/10230/70997
• dc.language.iso eng
• dc.publisher American Chemical Society (ACS)
• dc.relation.ispartof ACS Synth Biol. 2025 Jul 18;14(7):2797-809
• dc.rights © 2025 The Authors. Published by American Chemical Society. This publication is licensed under CC-BY 4.0 (http://creativecommons.org/licenses/by/4.0/).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Dinoroseobacter shibae
• dc.subject.keyword ELMs
• dc.subject.keyword Biofilm
• dc.subject.keyword Biosensors
• dc.subject.keyword Marine bacteria
• dc.subject.keyword Surface colonization
• dc.title Engineered marine biofilms for ocean environment monitoring
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion