Chromosome fissions and fusions act as barriers to gene flow between Brenthis Fritillary butterflies

Mostra el registre complet Registre parcial de l'ítem

• dc.contributor.author Mackintosh, Alexander
• dc.contributor.author Vila, Roger
• dc.contributor.author Laetsch, Dominik R.
• dc.contributor.author Hayward, Alexander
• dc.contributor.author Martin, Simon H.
• dc.contributor.author Lohse, Konrad
• dc.date.accessioned 2023-06-12T05:42:24Z
• dc.date.available 2023-06-12T05:42:24Z
• dc.date.issued 2023
• dc.description Includes supplementary materials for the online appendix.
• dc.description.abstract Chromosome rearrangements are thought to promote reproductive isolation between incipient species. However, it is unclear how often, and under what conditions, fission and fusion rearrangements act as barriers to gene flow. Here we investigate speciation between two largely sympatric fritillary butterflies, Brenthis daphne and Brenthis ino. We use a composite likelihood approach to infer the demographic history of these species from whole-genome sequence data. We then compare chromosome-level genome assemblies of individuals from each species and identify a total of nine chromosome fissions and fusions. Finally, we fit a demographic model where effective population sizes and effective migration rate vary across the genome, allowing us to quantify the effects of chromosome rearrangements on reproductive isolation. We show that chromosomes involved in rearrangements experienced less effective migration since the onset of species divergence and that genomic regions near rearrangement points have a further reduction in effective migration rate. Our results suggest that the evolution of multiple rearrangements in the B. daphne and B. ino populations, including alternative fusions of the same chromosomes, have resulted in a reduction in gene flow. Although fission and fusion of chromosomes are unlikely to be the only processes that have led to speciation between these butterflies, this study shows that these rearrangements can directly promote reproductive isolation and may be involved in speciation when karyotypes evolve quickly.
• dc.description.sponsorship We would like to thank Marian Thompson and Robert Foster (both Edinburgh Genomics) for preparing Pacbio and HiC sequencing libraries and Katy MacDonald for help in the molecular lab. We also thank Maria Jesus Cañal Villanueva and Luis Valledor (Universidad de Orviedo) for help with fieldwork logistics, as well as Vlad Dincă, Raluca Vodă, and Sabina Vila for contributing samples. We would like to thank Staffan Bensch and Deborah Charlesworth for insightful comments on an earlier version of the manuscript and Sam Ebdon for helping to improve figure 1. A.M. is supported by an E4 PhD studentship from the Natural Environment Research Council (NE/S007407/1). K.L. is supported by a fellowship from the Natural Environment Research Council (NERC, NE/L011522/1). R.V. is supported by Grant PID2019-107078GB-I00 funded by Ministerio de Ciencia e Innovación and Agencia Estatal de Investigación (MCIN/AEI/10.13039/501100011033). S.H.M. is supported by a Royal Society University Research Fellowship (URF/R1/180682). This work was supported by a European Research Council starting grant (ModelGenomLand 757648) to K.L. and a David Phillips Fellowship (BB/N020146/1) by the Biotechnology and Biological Sciences Research Council (BBSRC) to A.H.
• dc.format.mimetype application/pdf
• dc.identifier.citation Mackintosh A, Vila R, Laetsch DR, Hayward A, Martin SH, Lohse K. Chromosome fissions and fusions act as barriers to gene flow between Brenthis Fritillary butterflies. Molecular Biology and Evolution. 2023 Mar;40(3):msad043. DOI: 10.1093/molbev/msad043
• dc.identifier.doi http://dx.doi.org/10.1093/molbev/msad043
• dc.identifier.issn 1537-1719
• dc.identifier.uri http://hdl.handle.net/10230/57146
• dc.language.iso eng
• dc.publisher Oxford University Press
• dc.relation.ispartof Molecular Biology and Evolution. 2023 Mar;40(3):msad043
• dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/757648
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PID2019-107078GB-I00
• dc.rights © The Author(s) 2023. Published by Oxford University Press on behalf of Society for Molecular Biology and Evolution. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Chromosome evolution
• dc.subject.keyword Speciation
• dc.subject.keyword Population genomics
• dc.subject.keyword Chromosome rearrangements
• dc.subject.keyword Demographic inference
• dc.title Chromosome fissions and fusions act as barriers to gene flow between Brenthis Fritillary butterflies
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion