Teleost IgD + IgM - B cells mount clonally expanded and mildly mutated intestinal IgD responses in the absence of lymphoid follicles
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- dc.contributor.author Perdiguero, Pedro
- dc.contributor.author Martín-Martín, Alba
- dc.contributor.author Benedicenti, Ottavia
- dc.contributor.author Díaz-Rosales, Patricia
- dc.contributor.author Morel, Esther
- dc.contributor.author Muñoz-Atienza, Estefanía
- dc.contributor.author García-Flores, Mónica
- dc.contributor.author Simón, Rocío
- dc.contributor.author Soleto, Irene
- dc.contributor.author Cerutti, Andrea, 1965-
- dc.contributor.author Tafalla, Carolina
- dc.date.accessioned 2021-03-24T07:32:57Z
- dc.date.available 2021-03-24T07:32:57Z
- dc.date.issued 2019
- dc.description.abstract Immunoglobulin D (IgD) is an ancient antibody with dual membrane-bound and fluid-phase antigen receptor functions. The biology of secreted IgD remains elusive. Here, we demonstrate that teleost IgD+IgM- plasmablasts constitute a major lymphocyte population in some mucosal surfaces, including the gut mucosa. Remarkably, secreted IgD binds to gut commensal bacteria, which in turn stimulate IgD gene transcription in gut B cells. Accordingly, secreted IgD from gut as well as gill mucosae, but not the spleen, show a V(D)J gene configuration consistent with microbiota-driven clonal expansion and diversification, including mild somatic hypermutation. By showing that secreted IgD establishes a mutualistic relationship with commensals, our findings suggest that secreted IgD may play an evolutionary conserved role in mucosal homeostasis.
- dc.description.sponsorship Lucia González and Beatriz Abós are greatly acknowledged for technical assistance. This work was supported by the European Research Council (ERC Consolidator Grant 725061), the Spanish Ministry of Science, Innovation, and Universities (project AGL2017-85494-C2-1-R), and the Comunidad de Madrid (grant 2016-T1/BIO-1672).
- dc.format.mimetype application/pdf
- dc.identifier.citation Perdiguero P, Martín-Martín A, Benedicenti O, Díaz-Rosales P, Morel E, Muñoz-Atienza E, et al. Teleost IgD + IgM - B cells mount clonally expanded and mildly mutated intestinal IgD responses in the absence of lymphoid follicles. Cell Rep. 2019 Dec 24; 29(13): 4223-35.e5. DOI: 10.1016/j.celrep.2019.11.101
- dc.identifier.doi http://dx.doi.org/10.1016/j.celrep.2019.11.101
- dc.identifier.issn 2211-1247
- dc.identifier.uri http://hdl.handle.net/10230/46914
- dc.language.iso eng
- dc.publisher Elsevier
- dc.relation.ispartof Cell Reports. 2019 Dec 24;29(13):4223-35
- dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/725061
- dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/AGL2017-85494-C2-1-R
- dc.rights Copyright © 2019 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
- dc.subject.keyword B cells
- dc.subject.keyword IgD
- dc.subject.keyword SHM
- dc.subject.keyword Clonal expansion
- dc.subject.keyword Gills
- dc.subject.keyword Intestine
- dc.subject.keyword Microbiota
- dc.subject.keyword Somatic hypermutation
- dc.subject.keyword Teleost fish
- dc.title Teleost IgD + IgM - B cells mount clonally expanded and mildly mutated intestinal IgD responses in the absence of lymphoid follicles
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion