Teleost IgD + IgM - B cells mount clonally expanded and mildly mutated intestinal IgD responses in the absence of lymphoid follicles

Perdiguero, Pedro; Martín-Martín, Alba; Benedicenti, Ottavia; Díaz-Rosales, Patricia; Morel, Esther; Muñoz-Atienza, Estefanía; García-Flores, Mónica; Simón, Rocío; Soleto, Irene; Cerutti, Andrea, 1965-; Tafalla, Carolina

Teleost IgD + IgM - B cells mount clonally expanded and mildly mutated intestinal IgD responses in the absence of lymphoid follicles

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dc.contributor.author Perdiguero, Pedro
dc.contributor.author Martín-Martín, Alba
dc.contributor.author Benedicenti, Ottavia
dc.contributor.author Díaz-Rosales, Patricia
dc.contributor.author Morel, Esther
dc.contributor.author Muñoz-Atienza, Estefanía
dc.contributor.author García-Flores, Mónica
dc.contributor.author Simón, Rocío
dc.contributor.author Soleto, Irene
dc.contributor.author Cerutti, Andrea, 1965-
dc.contributor.author Tafalla, Carolina
dc.date.accessioned 2021-03-24T07:32:57Z
dc.date.available 2021-03-24T07:32:57Z
dc.date.issued 2019
dc.description.abstract Immunoglobulin D (IgD) is an ancient antibody with dual membrane-bound and fluid-phase antigen receptor functions. The biology of secreted IgD remains elusive. Here, we demonstrate that teleost IgD+IgM- plasmablasts constitute a major lymphocyte population in some mucosal surfaces, including the gut mucosa. Remarkably, secreted IgD binds to gut commensal bacteria, which in turn stimulate IgD gene transcription in gut B cells. Accordingly, secreted IgD from gut as well as gill mucosae, but not the spleen, show a V(D)J gene configuration consistent with microbiota-driven clonal expansion and diversification, including mild somatic hypermutation. By showing that secreted IgD establishes a mutualistic relationship with commensals, our findings suggest that secreted IgD may play an evolutionary conserved role in mucosal homeostasis.
dc.description.sponsorship Lucia González and Beatriz Abós are greatly acknowledged for technical assistance. This work was supported by the European Research Council (ERC Consolidator Grant 725061), the Spanish Ministry of Science, Innovation, and Universities (project AGL2017-85494-C2-1-R), and the Comunidad de Madrid (grant 2016-T1/BIO-1672).
dc.format.mimetype application/pdf
dc.identifier.citation Perdiguero P, Martín-Martín A, Benedicenti O, Díaz-Rosales P, Morel E, Muñoz-Atienza E, et al. Teleost IgD + IgM - B cells mount clonally expanded and mildly mutated intestinal IgD responses in the absence of lymphoid follicles. Cell Rep. 2019 Dec 24; 29(13): 4223-35.e5. DOI: 10.1016/j.celrep.2019.11.101
dc.identifier.doi http://dx.doi.org/10.1016/j.celrep.2019.11.101
dc.identifier.issn 2211-1247
dc.identifier.uri http://hdl.handle.net/10230/46914
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartof Cell Reports. 2019 Dec 24;29(13):4223-35
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/725061
dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/AGL2017-85494-C2-1-R
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.keyword B cells
dc.subject.keyword IgD
dc.subject.keyword SHM
dc.subject.keyword Clonal expansion
dc.subject.keyword Gills
dc.subject.keyword Intestine
dc.subject.keyword Microbiota
dc.subject.keyword Somatic hypermutation
dc.subject.keyword Teleost fish
dc.title Teleost IgD + IgM - B cells mount clonally expanded and mildly mutated intestinal IgD responses in the absence of lymphoid follicles
dc.type info:eu-repo/semantics/article
dc.type.version info:eu-repo/semantics/publishedVersion

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