Transcriptomic microRNA profiling of dendritic cells in response to gut microbiota-secreted vesicles

Mostra el registre complet Registre parcial de l'ítem

• dc.contributor.author Díaz Garrido, Natalia
• dc.contributor.author Bonnin, Sarah
• dc.contributor.author Riera-Borrull, Marta
• dc.contributor.author Giménez, Rosa
• dc.contributor.author Badia, Josefa
• dc.contributor.author Baldomà, Laura
• dc.date.accessioned 2020-10-14T05:57:35Z
• dc.date.available 2020-10-14T05:57:35Z
• dc.date.issued 2020
• dc.description.abstract The interconnection between nutrients, metabolites and microbes is a key factor governing the healthy/pathological status of an individual. Thus, microbiota-based research is essential in order to better understand human health and nutrition. Gut bacteria release membrane vesicles (MVs) as an intercellular communication mechanism that allows the direct delivery of factors that prime the host's innate immune system. We have previously shown that MVs from intestinal E. coli activate dendritic cells (DCs) in a strain-specific manner. To gain insights into the regulatory mechanisms involved, here, we have used an RNA deep sequencing approach to identify differentially expressed miRNAs (microRNAs) in DCs which are challenged by the MVs of the probiotic Nissle 1917 (EcN) or the commensal ECOR12. MicroRNAs are post-transcriptional regulatory mediators that permit the fine tuning of signaling pathways. This approach allowed the identification of a common set of miRNAs which are modulated by MVs from both strains and miRNAs which are differentially expressed in response to EcN or ECOR12 MVs. Based on the differential expression of the target genes and subsequent validation experiments, we correlated some of the selected miRNAs with the reported cytokine profile and specific T cell responses. As far as we know, this is the first study to analyze the regulation of miRNAs in DCs by MVs released by gut microbiota.
• dc.format.mimetype application/pdf
• dc.identifier.citation Díaz-Garrido N, Bonnin S, Riera M, Gíménez R, Badia J, Baldomà L. Transcriptomic microRNA profiling of dendritic cells in response to gut microbiota-secreted vesicles. Cells. 2020; 9(6):1534. DOI: 10.3390/cells9061534
• dc.identifier.doi http://dx.doi.org/10.3390/cells9061534
• dc.identifier.issn 2073-4409
• dc.identifier.uri http://hdl.handle.net/10230/45478
• dc.language.iso eng
• dc.publisher MDPI
• dc.relation.ispartof Cells. 2020; 9(6):1534
• dc.rights © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword E. coli Nissle 1917
• dc.subject.keyword Bacterial extracellular vesicles
• dc.subject.keyword Gene expression
• dc.subject.keyword Immune modulation
• dc.subject.keyword Intestinal homeostasis
• dc.subject.keyword miRNAs
• dc.subject.keyword Microbiota–host communication
• dc.subject.keyword Probiotics
• dc.title Transcriptomic microRNA profiling of dendritic cells in response to gut microbiota-secreted vesicles
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion