A Sprouty4 reporter to monitor FGF/ERK signaling activity in ESCs and mice

Morgani, Sophie M.; Saiz, Néstor; Garg, Vidur; Raina, Dhruv; Simon, Claire S.; Kang, Minjung; Martínez Arias, Alfonso; Nichols, Jennifer; Schröter, Christian; Hadjantonakis, Anna-Katerina

A Sprouty4 reporter to monitor FGF/ERK signaling activity in ESCs and mice

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dc.contributor.author Morgani, Sophie M.
dc.contributor.author Saiz, Néstor
dc.contributor.author Garg, Vidur
dc.contributor.author Raina, Dhruv
dc.contributor.author Simon, Claire S.
dc.contributor.author Kang, Minjung
dc.contributor.author Martínez Arias, Alfonso
dc.contributor.author Nichols, Jennifer
dc.contributor.author Schröter, Christian
dc.contributor.author Hadjantonakis, Anna-Katerina
dc.date.accessioned 2023-04-25T06:14:35Z
dc.date.available 2023-04-25T06:14:35Z
dc.date.issued 2018
dc.description.abstract The FGF/ERK signaling pathway is highly conserved throughout evolution and plays fundamental roles during embryonic development and in adult organisms. While a plethora of expression data exists for ligands, receptors and pathway regulators, we know little about the spatial organization or dynamics of signaling in individual cells within populations. To this end we developed a transcriptional readout of FGF/ERK activity by targeting a histone H2B-linked Venus fluorophore to the endogenous locus of Spry4, an early pathway target, and generated Spry4H2B-Venus embryonic stem cells (ESCs) and a derivative mouse line. The Spry4H2B-Venus reporter was heterogeneously expressed within ESC cultures and responded to FGF/ERK signaling manipulation. In vivo, the Spry4H2B-Venus reporter recapitulated the expression pattern of Spry4 and localized to sites of known FGF/ERK activity including the inner cell mass of the pre-implantation embryo and the limb buds, somites and isthmus of the post-implantation embryo. Additionally, we observed highly localized reporter expression within adult organs. Genetic and chemical disruption of FGF/ERK signaling, in vivo in pre- and post-implantation embryos, abrogated Venus expression establishing the reporter as an accurate signaling readout. This tool will provide new insights into the dynamics of the FGF/ERK signaling pathway during mammalian development.
dc.format.mimetype application/pdf
dc.identifier.citation Morgani SM, Saiz N, Garg V, Raina D, Simon CS, Kang M, Arias AM, Nichols J, Schröter C, Hadjantonakis AK. A Sprouty4 reporter to monitor FGF/ERK signaling activity in ESCs and mice. Dev Biol. 2018 Sep 1;441(1):104-26. DOI: 10.1016/j.ydbio.2018.06.017
dc.identifier.doi http://dx.doi.org/10.1016/j.ydbio.2018.06.017
dc.identifier.issn 0012-1606
dc.identifier.uri http://hdl.handle.net/10230/56561
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartof Dev Biol. 2018 Sep 1;441(1):104-26
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.subject.keyword ESCs
dc.subject.keyword FGF signaling
dc.subject.keyword FGF/ERK
dc.subject.keyword Fluorescent reporter
dc.subject.keyword Live imaging
dc.subject.keyword Mouse embryo
dc.subject.keyword Quantitative image analysis
dc.subject.keyword Sprouty4
dc.title A Sprouty4 reporter to monitor FGF/ERK signaling activity in ESCs and mice
dc.type info:eu-repo/semantics/article
dc.type.version info:eu-repo/semantics/acceptedVersion

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