Comparative analysis of miRNA expression during the development of insects of different metamorphosis modes and germband types

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• dc.contributor.author Ylla Bou, Guillem, 1990-ca
• dc.contributor.author Piulachs i Bagà, M. Dolorsca
• dc.contributor.author Bellés, Xavier, 1952-ca
• dc.date.accessioned 2018-08-29T08:57:19Z
• dc.date.available 2018-08-29T08:57:19Z
• dc.date.issued 2017
• dc.description.abstract BACKGROUND: Do miRNAs contribute to specify the germ-band type and the body structure in the insect embryo? Our goal was to address that issue by studying the changes in miRNA expression along the ontogeny of the German cockroach Blattella germanica, which is a short germ-band and hemimetabolan species. RESULTS: We sequenced small RNA libraries representing 11 developmental stages of B. germanica ontogeny (with especial emphasis on embryogenesis) and the changes in miRNA expression were examined. Data were compared with equivalent data for two long germ-band holometabolan species Drosophila melanogaster and Drosophila virilis, and the short germ-band holometabolan species Tribolium castaneum. The identification of B. germanica embryo small RNA sequences unveiled miRNAs not detected in previous studies, such as those of the MIR-309 family and 54 novel miRNAs. Four main waves of miRNA expression were recognized (with most miRNA changes occurring during the embryonic stages): the first from day 0 to day 1 of embryogenesis, the second during mid-embryogenesis (days 0-6), the third (with an acute expression peak) on day 2 of embryonic development, and the fourth during post-embryonic development. The second wave defined the boundaries of maternal-to-zygotic transition, with maternal mRNAs being cleared, presumably by Mir-309 and associated scavenger miRNAs. CONCLUSION: miRNAs follow well-defined patterns of expression over hemimetabolan ontogeny, patterns that are more diverse during embryonic development than during the nymphal stages. The results suggest that miRNAs play important roles in the developmental transitions between the embryonic stages of development (starting with maternal loading), during which they might influence the germ-band type and metamorphosis mode.
• dc.description.sponsorship This work was supported by the Spanish Ministry of Economy and Competitiveness (grants CGL2012–36251 and CGL2015–64727-P to X.B.), the Spanish Ministry of Science and Innovation (grants BFU2011–22404 and CGL2016–76011-R to M.D.P.) and the Catalan Government (2014 SGR 619). It also received financial assistance from the European Fund for Economic and Regional Development (FEDER funds to X.B. and M.D.P.)
• dc.format.mimetype application/pdf
• dc.identifier.citation Ylla G, Piulachs MD, Belles X. Comparative analysis of miRNA expression during the development of insects of different metamorphosis modes and germband types. BMC genomics. 2017 Oct 11;18(1):774. DOI: 10.1186/s12864-017-4177-5
• dc.identifier.doi http://dx.doi.org/10.1186/s12864-017-4177-5
• dc.identifier.issn 1471-2164
• dc.identifier.uri http://hdl.handle.net/10230/35403
• dc.language.iso eng
• dc.publisher BioMed Centralca
• dc.relation.ispartof BMC genomics. 2017 Oct 11;18(1):774
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/CGL2012–36251
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/CGL2015–64727-P
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2011–22404
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/CGL2016–76011-R
• dc.rights © Guillem Ylla, Maria-Dolors Piulachs and Xavier Belles 2017. Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.other Blàstula
• dc.subject.other Blatèlids -- Genètica
• dc.subject.other Expressió gènica
• dc.subject.other Metamorfosi
• dc.subject.other MicroARN
• dc.title Comparative analysis of miRNA expression during the development of insects of different metamorphosis modes and germband typesca
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion