Length and repeat-sequence variation in 58 STRs and 94 SNPs in two Spanish populations

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• dc.contributor.author Casals López, Ferranca
• dc.contributor.author Anglada Busquets, Rogerca
• dc.contributor.author Bonet, Núriaca
• dc.contributor.author Rasal Soteras, Raquelca
• dc.contributor.author van der Gaag, Kristiaan J.ca
• dc.contributor.author Hoogenboom, Jerryca
• dc.contributor.author Solé Morata, Neus, 1988-ca
• dc.contributor.author Comas, David, 1969-ca
• dc.contributor.author Calafell i Majó, Francescca
• dc.date.accessioned 2017-06-26T10:10:46Z
• dc.date.issued 2017
• dc.description.abstract We have genotyped the 58 STRs (27 autosomal, 24 Y-STRs and 7 X-STRs) and 94 autosomal SNPs in Illumina ForenSeq™ Primer Mix A in 88 Spanish Roma (Gypsy) samples and 143 Catalans. Since this platform is based in massive parallel sequencing, we have used simple R scripts to uncover the sequence variation in the repeat region. Thus, we have found, across 58 STRs, 541 length-based alleles, which, after considering repeat-sequence variation, became 804 different alleles. All loci in both populations were in Hardy-Weinberg equilibrium. FST between both populations was 0.0178 for autosomal SNPs, 0.0146 for autosomal STRs, 0.0101 for X-STRs and 0.1866 for Y-STRs. Combined a priori statistics showed quite large; for instance, pooling all the autosomal loci, the a priori probabilities of discriminating a suspect become 1 − (2.3 × 10−70) and 1 − (5.9 × 10−73), for Roma and Catalans respectively, and the chances of excluding a false father in a trio are 1 − (2.6 × 10−20) and 1 − (2.0 × 10−21).
• dc.description.sponsorship Funding was provided by the Spanish Agencia Estatal de Investigación (AEI) and Fondo Europeo de Desarollo Regional (FEDER) (grant CGL2016-75389-P), and by Agència de Gestió d’Ajuts Universitaris i de la Recerca (Generalitat de Catalunya) grant 2014 SGR 866.
• dc.format.mimetype application/pdfca
• dc.identifier.citation Casals López F, Anglada Busquets R, Bonet N, Rasal Soteras R, van der Gaag KJ, Hoogenboom J et al. Length and repeat-sequence variation in 58 STRs and 94 SNPs in two Spanish populations. Forensic Science International: Genetics. 2017;30:66-70. DOI: 10.1016/j.fsigen.2017.06.006
• dc.identifier.doi http://dx.doi.org/10.1016/j.fsigen.2017.06.006
• dc.identifier.issn 1872-4973
• dc.identifier.uri http://hdl.handle.net/10230/32467
• dc.language.iso eng
• dc.publisher Elsevierca
• dc.relation.ispartof Forensic Science International: Genetics. 2017;30:66-70
• dc.rights © Elsevier http://dx.doi.org/10.1016/j.fsigen.2017.06.006
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.subject.keyword Massive parallel sequencing
• dc.subject.keyword Repeat sequence-based alleles
• dc.subject.keyword Roma
• dc.subject.keyword Catalans
• dc.title Length and repeat-sequence variation in 58 STRs and 94 SNPs in two Spanish populationsca
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/acceptedVersion