Circulating miRNAs, isomiRs and small RNA clusters in human plasma and breast milk
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- dc.contributor.author Rubio Martínez, Mercedes, 1980-
- dc.contributor.author Bustamante Pineda, Mariona
- dc.contributor.author Hernandez-Ferrer, Carles, 1987-
- dc.contributor.author Fernández-Orth, Dietmar
- dc.contributor.author Pantano Rubiño, Lorena, 1982-
- dc.contributor.author Sarria-Trujillo, Yaris
- dc.contributor.author Piqué-Borras, Maria
- dc.contributor.author Vellve del Amo, Kilian
- dc.contributor.author Agramunt, Silvia
- dc.contributor.author Carreras Collado, Ramón
- dc.contributor.author Estivill, Xavier, 1955-
- dc.contributor.author González Ruiz, Juan Ramón
- dc.contributor.author Mayor, Alfredo
- dc.date.accessioned 2019-01-16T08:09:58Z
- dc.date.available 2019-01-16T08:09:58Z
- dc.date.issued 2018
- dc.description.abstract Circulating small RNAs, including miRNAs but also isomiRs and other RNA species, have the potential to be used as non-invasive biomarkers for communicable and non-communicable diseases. This study aims to characterize and compare small RNA profiles in human biofluids. For this purpose, RNA was extracted from plasma and breast milk samples from 15 healthy postpartum mothers. Small RNA libraries were prepared with the NEBNext® small RNA library preparation kit and sequenced in an Illumina HiSeq2000 platform. miRNAs, isomiRs and clusters of small RNAs were annotated using seqBuster/seqCluster framework in 5 plasma and 10 milk samples that passed the initial quality control. The RNA yield was 81 ng/mL [standard deviation (SD): 41] and 3985 ng/mL (SD: 3767) for plasma and breast milk, respectively. Mean number of good quality reads was 4.04 million (M) (40.01% of the reads) in plasma and 12.5M (89.6%) in breast milk. One thousand one hundred eighty two miRNAs, 12,084 isomiRs and 1,053 small RNA clusters that included piwi-interfering RNAs (piRNAs), tRNAs, small nucleolar RNAs (snoRNA) and small nuclear RNAs (snRNAs) were detected. Samples grouped by biofluid, with 308 miRNAs, 1,790 isomiRs and 778 small RNA clusters differentially detected. In summary, plasma and milk showed a different small RNA profile. In both, miRNAs, piRNAs, tRNAs, snRNAs, and snoRNAs were identified, confirming the presence of non-miRNA species in plasma, and describing them for the first time in milk.
- dc.format.mimetype application/pdf
- dc.identifier.citation Rubio M, Bustamante M, Hernandez-Ferrer C, Fernandez-Orth D, Pantano L, Sarria Y. et al. Circulating miRNAs, isomiRs and small RNA clusters in human plasma and breast milk. PLoS One. 2018 Mar 5;13(3):e0193527. DOI: 10.1371/journal.pone.0193527
- dc.identifier.doi http://dx.doi.org/10.1371/journal.pone.0193527
- dc.identifier.issn 1932-6203
- dc.identifier.uri http://hdl.handle.net/10230/36282
- dc.language.iso eng
- dc.publisher Public Library of Science (PLoS)
- dc.relation.ispartof PLoS One. 2018 Mar 5;13(3):e0193527
- dc.rights Copyright © 2018 Rubio et al. This is an open access article distributed under the terms of the Creative Commons Attribution License https://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri https://creativecommons.org/licenses/by/4.0/
- dc.subject.other Llet materna -- Investigació
- dc.subject.other MicroRNAs
- dc.title Circulating miRNAs, isomiRs and small RNA clusters in human plasma and breast milk
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion