Human tRNAs with inosine 34 are essential to efficiently translate eukarya-specific low-complexity proteins

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  • dc.contributor.author Torres, Adrian Gabriel
  • dc.contributor.author Rodríguez-Escribà, Marta
  • dc.contributor.author Marcet Houben, Marina
  • dc.contributor.author Santos Vieira, Helaine Graziele
  • dc.contributor.author Camacho, Noelia
  • dc.contributor.author Catena, Helena
  • dc.contributor.author Murillo Recio, Marina
  • dc.contributor.author Rafels-Ybern, Àlbert
  • dc.contributor.author Reina García, Óscar, 1976-
  • dc.contributor.author Torres, Francisco Miguel
  • dc.contributor.author Pardo Saganta, Ana
  • dc.contributor.author Gabaldón Estevan, Juan Antonio, 1973-
  • dc.contributor.author Novoa, Eva Maria
  • dc.contributor.author Ribas de Pouplana, Lluís
  • dc.date.accessioned 2021-12-13T10:48:17Z
  • dc.date.available 2021-12-13T10:48:17Z
  • dc.date.issued 2021
  • dc.description.abstract The modification of adenosine to inosine at the wobble position (I34) of tRNA anticodons is an abundant and essential feature of eukaryotic tRNAs. The expansion of inosine-containing tRNAs in eukaryotes followed the transformation of the homodimeric bacterial enzyme TadA, which generates I34 in tRNAArg and tRNALeu, into the heterodimeric eukaryotic enzyme ADAT, which modifies up to eight different tRNAs. The emergence of ADAT and its larger set of substrates, strongly influenced the tRNA composition and codon usage of eukaryotic genomes. However, the selective advantages that drove the expansion of I34-tRNAs remain unknown. Here we investigate the functional relevance of I34-tRNAs in human cells and show that a full complement of these tRNAs is necessary for the translation of low-complexity protein domains enriched in amino acids cognate for I34-tRNAs. The coding sequences for these domains require codons translated by I34-tRNAs, in detriment of synonymous codons that use other tRNAs. I34-tRNA-dependent low-complexity proteins are enriched in functional categories related to cell adhesion, and depletion in I34-tRNAs leads to cellular phenotypes consistent with these roles. We show that the distribution of these low-complexity proteins mirrors the distribution of I34-tRNAs in the phylogenetic tree.
  • dc.description.sponsorship Funding: Spanish Ministry of Economy and Competitiveness [PID2019-108037RB-100 to L.R.d.P., PGC2018-099921 to T.G., PGC2018-098152-A-100 to E.M.N]; Australian Research Council [DP180103571 to E.M.N]; European Union's Horizon 2020 research and innovation programme [ERC-2016–724173 to T.G.]. Funding for open access charge: Spanish Ministry of Economy and Competitiveness
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Gabriel Torres A, Rodríguez-Escribà M, Marcet-Houben M, Santos Vieira HG, Camacho N, Catena H et al. Human tRNAs with inosine 34 are essential to efficiently translate eukarya-specific low-complexity proteins. Nucleic Acids Res. 2021 Jul 9;49(12):7011-34. DOI: 10.1093/nar/gkab461
  • dc.identifier.doi http://dx.doi.org/10.1093/nar/gkab461
  • dc.identifier.issn 0305-1048
  • dc.identifier.uri http://hdl.handle.net/10230/49182
  • dc.language.iso eng
  • dc.publisher Oxford University Press
  • dc.relation.ispartof Nucleic Acids Research. 2021 Jul 9;49(12):7011-34.
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/724173
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PGC2018-098152-A-100
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PID2019-108037RB-100
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PGC2018-099921
  • dc.rights © Adrian Gabriel Torres et al. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri https://creativecommons.org/licenses/by/4.0/
  • dc.subject.other Genètica
  • dc.subject.other Proteïnes
  • dc.subject.other Enzims
  • dc.title Human tRNAs with inosine 34 are essential to efficiently translate eukarya-specific low-complexity proteins
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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