A chromosome‐level genome assembly enables the identification of the follicule stimulating hormone receptor as the master sex‐determining gene in the flatfish Solea senegalensis

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• dc.contributor.author de la Herrán, Roberto
• dc.contributor.author Gómez-Garrido, Jèssica
• dc.contributor.author Cruz, Fernando
• dc.contributor.author Alioto, Tyler
• dc.contributor.author Martínez, Paulino
• dc.date.accessioned 2023-03-20T07:23:59Z
• dc.date.available 2023-03-20T07:23:59Z
• dc.date.issued 2023
• dc.description Includes supplementary materials for the online appendix.
• dc.description.abstract Sex determination (SD) shows huge variation among fish and a high evolutionary rate, as illustrated by the Pleuronectiformes (flatfishes). This order is characterized by its adaptation to demersal life, compact genomes and diversity of SD mechanisms. Here, we assembled the Solea senegalensis genome, a flatfish of great commercial value, into 82 contigs (614 Mb) combining long- and short-read sequencing, which were next scaffolded using a highly dense genetic map (28,838 markers, 21 linkage groups), representing 98.9% of the assembly. Further, we established the correspondence between the assembly and the 21 chromosomes by using BAC-FISH. Whole genome resequencing of six males and six females enabled the identification of 41 single nucleotide polymorphism variants in the follicle stimulating hormone receptor (fshr) consistent with an XX/XY SD system. The observed sex association was validated in a broader independent sample, providing a novel molecular sexing tool. The fshr gene displayed differential expression between male and female gonads from 86 days post-fertilization, when the gonad is still an undifferentiated primordium, concomitant with the activation of amh and cyp19a1a, testis and ovary marker genes, respectively, in males and females. The Y-linked fshr allele, which included 24 nonsynonymous variants and showed a highly divergent 3D protein structure, was overexpressed in males compared to the X-linked allele at all stages of gonadal differentiation. We hypothesize a mechanism hampering the action of the follicle stimulating hormone driving the undifferentiated gonad toward testis.
• dc.description.sponsorship This study was supported by the Spanish Ministry of Economy and Competitiveness, FEDER Grants (RTI2018-096847-B-C21, RTI2018-096847-B-C22 and RTI2018-097110-B-C21), Junta de Andalucía-FEDER Grant (P20-00938) and the European Union's Horizon 2020 research and innovation programme under grant agreement No. 817923 (AQUA-FAANG). We thank Geneaqua SL for their participation and financial support of sequencing. We acknowledge the bioinformatic support of the Centro de Supercomputación de Galicia (CESGA).
• dc.format.mimetype application/pdf
• dc.identifier.citation de la Herrán R, Hermida M, Rubiolo JA, Gómez‐Garrido J, Cruz F, Robles F, et al. A chromosome‐level genome assembly enables the identification of the follicule stimulating hormone receptor as the master sex‐determining gene in the flatfish Solea senegalensis. Molecular Ecology Resources. 2023 May;23(4):886-904. DOI: 10.1111/1755-0998.13750
• dc.identifier.doi http://dx.doi.org/10.1111/1755-0998.13750
• dc.identifier.issn 1755-098X
• dc.identifier.uri http://hdl.handle.net/10230/56273
• dc.language.iso eng
• dc.publisher Wiley
• dc.relation.ispartof Molecular Ecology Resources. 2023 May;23(4):886-904
• dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/817923
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/RTI2018-096847-B-C21
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/RTI2018-096847-B-C22
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/RTI2018-097110-B-C21
• dc.rights © 2023 The Authors. Molecular Ecology Resources published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
• dc.subject.keyword Follicule stimulating hormone receptor
• dc.subject.keyword Genetic map
• dc.subject.keyword Senegalese sole
• dc.subject.keyword Sex determination
• dc.subject.keyword Whole genome sequencing
• dc.title A chromosome‐level genome assembly enables the identification of the follicule stimulating hormone receptor as the master sex‐determining gene in the flatfish Solea senegalensis
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion