Diagnostic Accuracy of the Enhanced Liver Fibrosis (ELF®) Score Using HCV-Infected Serum Samples Cryopreserved for up to 25 Years
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- dc.contributor.author Puigvehí, Marcca
- dc.contributor.author Hernández, Juanjoca
- dc.contributor.author Broquetas, Teresaca
- dc.contributor.author Coll Estrada, Susannaca
- dc.contributor.author Garcia-Retortillo, Montserratca
- dc.contributor.author Cañete Hidalgo, Nuriaca
- dc.contributor.author Giménez Romaní, Maria Dolorsca
- dc.contributor.author Garcia, Marca
- dc.contributor.author Bory Ros, Felipeca
- dc.contributor.author Salvadó, Margaritaca
- dc.contributor.author Solà Lamoglia, Ricardca
- dc.contributor.author Carrión Rodríguez, José Antonioca
- dc.date.accessioned 2018-02-22T08:18:12Z
- dc.date.available 2018-02-22T08:18:12Z
- dc.date.issued 2016
- dc.description.abstract INTRODUCTION & AIMS: Cryopreservation of serum samples is a standard procedure for biomedical research in tertiary centers. However, studies evaluating the long-term biological stability of direct liver fibrosis markers using cryopreserved samples are scarce. METHODS: We compared the stability of hyaluronic acid (HA), tissue inhibitor of metalloproteinases (TIMP-1) and amino-terminal propeptide of type III procollagen (PIIINP) in 225 frozen serum samples of HCV-infected patients with a paired liver biopsy for up to 25 years (1990-2014). Moreover, we assessed the diagnostic accuracy (AUROC) of the Enhanced Liver Fibrosis (ELF®) score to identify significant fibrosis (F2-4) and its predictive capacity to identify clinical events during follow-up. RESULTS: Seventy-six patients (39,8%) had mild fibrosis (F0-1) and 115 (60,2%) significant fibrosis (F2-4). HA, PIIINP and TIMP-1 values remained stable during the period from 1995 to 2014 while those of 1990-94 were slightly higher. We did not find significant differences in the median ELF® values during the 20-year period from 1995-2014 in patients with mild (from 8,4 to 8,7) and significant fibrosis (from 9,9 to 10,9) (p = ns between periods and fibrosis stages). The AUROCs of ELF® to identify significant fibrosis were high in all the periods (from 0,85 to 0,91). The ELF® score showed a good predictive capability to identify clinical events during follow-up. CONCLUSIONS: The biological stability of direct serum markers (HA, PIIINP and TIMP-1) using HCV-infected samples cryopreserved for 20 years is good. Therefore, the diagnostic accuracy of the ELF® score to identify significant fibrosis and clinical events during follow-up is very high.
- dc.format.mimetype application/pdf
- dc.identifier.citation Puigvehí M, Hernández J, Broquetas T, Coll S, Garcia-Retortillo M, Cañete N. et al. Diagnostic Accuracy of the Enhanced Liver Fibrosis (ELF®) Score Using HCV-Infected Serum Samples Cryopreserved for up to 25 Years. PLoS One. 2016 Dec 16;11(12):e0164883. DOI: 10.1371/journal.pone.0164883
- dc.identifier.doi http://dx.doi.org/10.1371/journal.pone.0164883
- dc.identifier.issn 1932-6203
- dc.identifier.uri http://hdl.handle.net/10230/33968
- dc.language.iso eng
- dc.publisher Public Library of Science (PLoS)ca
- dc.relation.ispartof PLoS One. 2016 Dec 16;11(12):e0164883
- dc.rights © 2016 Marc Puigvehí et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.subject.other Fetge -- Malalties
- dc.subject.other Marcadors bioquímics
- dc.title Diagnostic Accuracy of the Enhanced Liver Fibrosis (ELF®) Score Using HCV-Infected Serum Samples Cryopreserved for up to 25 Yearsca
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion