Blastomeres of 8-cell mouse embryos differ in their ability to generate embryonic stem cells and produce lines with different transcriptional signatures

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  • dc.contributor.author Alonso-Alonso, Sandra
  • dc.contributor.author Esteve-Codina, Anna
  • dc.contributor.author Martín-Mur, Beatriz
  • dc.contributor.author Álvarez-González, Lucía
  • dc.contributor.author Ruiz Herrera, Aurora
  • dc.contributor.author Santaló, Josep
  • dc.contributor.author Ibáñez, Elena
  • dc.date.accessioned 2024-01-18T10:09:14Z
  • dc.date.available 2024-01-18T10:09:14Z
  • dc.date.issued 2023
  • dc.description.abstract Embryonic stem cell (ESC) derivation from single blastomeres of 8-cell mouse embryos results in lower derivation rates than that from whole blastocysts, raising a biological question about the developmental potential of sister blastomeres. We aimed to assess the ability of 8-cell blastomeres to produce epiblast cells and ESC lines after isolation, and the properties of the resulting lines. Our results revealed unequal competence among sister blastomeres to produce ESC lines. At least half of the blastomeres possess a lower potential to generate ESCs, although culture conditions and blastomeres plasticity can redirect their non-pluripotent fate towards the epiblast lineage, allowing us to generate up to seven lines from the same embryo. Lines originated from the same embryo segregated into two groups according to their transcriptional signatures. While the expression of genes related to pluripotency and development was higher in one group, no differences were found in their trilineage differentiation ability. These results may help to improve our understanding of the ESC derivation process from single blastomeres and cell fate determination in the preimplantation mouse embryos.
  • dc.description.sponsorship The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by Department of Research and Universities of the Generalitat de Catalunya (2021 SGR 00122) and the Spanish Ministry of Science and Innovation (PID2020-112557GB-I00 to AR-H). SA-A was beneficiary of a PIF-UAB fellowship and LA-G was supported by a FPI predoctoral fellowship from the Ministry of Economy and Competitiveness (PRE-2018-083257).
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Alonso-Alonso S, Esteve-Codina A, Martin-Mur B, Álvarez-González L, Ruiz-Herrera A, Santaló J, Ibáñez E. Blastomeres of 8-cell mouse embryos differ in their ability to generate embryonic stem cells and produce lines with different transcriptional signatures. Front Cell Dev Biol. 2023 Oct 9;11:1274660. DOI: 10.3389/fcell.2023.1274660
  • dc.identifier.doi http://dx.doi.org/10.3389/fcell.2023.1274660
  • dc.identifier.issn 2296-634X
  • dc.identifier.uri http://hdl.handle.net/10230/58750
  • dc.language.iso eng
  • dc.publisher Frontiers
  • dc.relation.ispartof Front Cell Dev Biol. 2023 Oct 9;11:1274660
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PID2020-112557GB-I00
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PRE2018-083257
  • dc.rights © 2023 Alonso-Alonso, Esteve-Codina, Martin-Mur, Álvarez-González, Ruiz-Herrera, Santaló and Ibáñez. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri http://creativecommons.org/licenses/by/4.0/
  • dc.subject.keyword ESCs
  • dc.subject.keyword Cell fate
  • dc.subject.keyword Developmental potential
  • dc.subject.keyword Epiblast
  • dc.subject.keyword Pluripotency
  • dc.title Blastomeres of 8-cell mouse embryos differ in their ability to generate embryonic stem cells and produce lines with different transcriptional signatures
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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