Taxonomic and functional fecal microbiota signatures associated with insulin resistance in non-diabetic subjects with overweight/obesity within the frame of the PREDIMED-Plus Study

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• dc.contributor.author Atzeni, Alessandro
• dc.contributor.author Bastiaanssen, Thomaz F.S.
• dc.contributor.author Cryan¸ John F.
• dc.contributor.author Tinahones Madueño, Francisco José
• dc.contributor.author Vioque, Jesus
• dc.contributor.author Corella, Dolores
• dc.contributor.author Fitó Colomer, Montserrat
• dc.contributor.author Vidal Alaball, Josep
• dc.contributor.author Moreno Indias, Isabel
• dc.contributor.author Gómez Pérez, Ana María
• dc.contributor.author Torres-Collado, Laura
• dc.contributor.author Coltell, Oscar
• dc.contributor.author Castañer, Olga
• dc.contributor.author Bulló, Mònica
• dc.contributor.author Salas Salvadó, Jordi
• dc.date.accessioned 2022-11-03T07:23:42Z
• dc.date.available 2022-11-03T07:23:42Z
• dc.date.issued 2022
• dc.description.abstract Objective: an altered gut microbiota has been associated with insulin resistance, a metabolic dysfunction consisting of cellular insulin signaling impairment. The aim of the present study is to determine the taxonomic and functional fecal microbiota signatures associated with HOMA-IR index in a population with high cardiovascular risk. Methods: a total of 279 non-diabetic individuals (55-75 years aged) with overweight/obesity and metabolic syndrome were stratified according to tertiles of HOMA-IR index. Blood biochemical parameters, anthropometric measurements and fecal samples were collected at baseline. Fecal microbial DNA extraction, 16S amplicon sequencing and bioinformatics analysis were performed. Results: desulfovibrio, Odoribacter and Oscillospiraceae UCG-002 were negatively associated with HOMA-IR index, whereas predicted total functional abundances revealed gut metabolic modules mainly linked to amino acid degradation. Butyricicoccus, Erysipelotrichaceae UCG-003, Faecalibacterium were positively associated with HOMA-IR index, whereas predicted total functional abundances revealed gut metabolic modules mainly linked to saccharide degradation. These bacteria contribute differentially to the gut metabolic modules, being the degree of contribution dependent on insulin resistance. Both taxa and gut metabolic modules negatively associated to HOMA-IR index were linked to mechanisms involving sulfate reducing bacteria, improvement of intestinal gluconeogenesis and production of acetate. Furthermore, both taxa and gut metabolic modules positively associated to HOMA-IR index were linked to production and mechanisms of action of butyrate. Conclusions: specific taxonomic and functional fecal microbiota signatures associated with insulin resistance were identified in a non-diabetic population with overweight/obesity at high cardiovascular risk. These findings suggest that tailoring therapies based on specific fecal microbiota profiles could be a potential strategy to improve insulin sensitivity.
• dc.description.sponsorship This research was funded by the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 713679 and from the Universitat Rovira i Virgili (URV). This work was supported by the official Spanish Institutions for funding scientific biomedical research, CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN) and Instituto de Salud Carlos III (ISCIII), through the Fondo de Investigación para la Salud (FIS), which is co-funded by the European Regional Development Fund (three coordinated FIS projects led by JJS, including the following projects: PI13/00462, PI16/00501 and PI19/00576); the Especial Action Project entitled: Implementación y evaluación de una intervención intensiva sobre la actividad física Cohorte PREDIMED-Plus grant to JSS; the Recercaixa (number 2013ACUP00194) grant to JSS; The Fondo de Investigaciones Sanitarias of the Instituto de Salut Carlos III PI17/00215; The Generalitat Valenciana PROMETEO 17/2017 and PROMETEO 21/2021. None of the funding sources took part in the design, collection, analysis, interpretation of the data, or writing the report, or in the decision to submit the manuscript for publication. JSS, senior author of this study gratefully acknowledges the financial support by ICREA under the ICREA Academia programme. Food companies Hojiblanca (Lucena, Spain) and Patrimonio Comunal Olivarero (Madrid, Spain) donated extra virgin olive oil; and the Almond Board of California (Modesto, CA), American Pistachio Growers (Fresno, CA), and Paramount Farms (Wonderful Company, LLC, Los Angeles, CA) donated nuts for the PREDIMED- Pilot.
• dc.format.mimetype application/pdf
• dc.identifier.citation Atzeni A, Bastiaanssen TFS, Cryan JF, Tinahones FJ, Vioque J, Corella D, et al. Taxonomic and functional fecal microbiota signatures associated with insulin resistance in non-diabetic subjects with overweight/obesity within the frame of the PREDIMED-Plus Study. Front Endocrinol (Lausanne). 2022 Apr 28; 13: 804455.DOI: 10.3389/fendo.2022.804455
• dc.identifier.doi http://dx.doi.org/10.3389/fendo.2022.804455
• dc.identifier.issn 1664-2392
• dc.identifier.uri http://hdl.handle.net/10230/54669
• dc.language.iso eng
• dc.publisher Frontiers
• dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/713679
• dc.rights Copyright © 2022 Atzeni, Bastiaanssen, Cryan, Tinahones, Vioque, Corella, Fitó, Vidal, Moreno-Indias, Gómez-Pérez, Torres-Collado, Coltell, Castañer, Bulló and Salas-Salvadó. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) http://creativecommons.org/licenses/by/4.0/. The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword 16S sequencing
• dc.subject.keyword HOMA-IR
• dc.subject.keyword Fecal microbiota
• dc.subject.keyword Gut metabolic modules
• dc.subject.keyword Insulin resisitance
• dc.title Taxonomic and functional fecal microbiota signatures associated with insulin resistance in non-diabetic subjects with overweight/obesity within the frame of the PREDIMED-Plus Study
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion