Comprehensive genome and epigenome characterization of CHO cells in response to evolutionary pressures and over time
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- dc.contributor.author Feichtinger, Juliaca
- dc.contributor.author Hernández, Inmaculadaca
- dc.contributor.author Fischer, Christophca
- dc.contributor.author Hanscho, Michaelca
- dc.contributor.author Auer, Norbertca
- dc.contributor.author Hackl, Matthiasca
- dc.contributor.author Jadhav, Vaibhavca
- dc.contributor.author Baumann, Martinaca
- dc.contributor.author Krempl, Peter M.ca
- dc.contributor.author Schmidl, Christianca
- dc.contributor.author Farlik, Matthiasca
- dc.contributor.author Schuster, Michaelca
- dc.contributor.author Merkel, Angelikaca
- dc.contributor.author Sommer, Andreasca
- dc.contributor.author Heath, Simonca
- dc.contributor.author Rico, Danielca
- dc.contributor.author Bock, Christophca
- dc.contributor.author Thallinger, Gerhard R.ca
- dc.contributor.author Borth, Nicoleca
- dc.date.accessioned 2017-02-08T08:17:49Z
- dc.date.available 2017-02-08T08:17:49Z
- dc.date.issued 2016ca
- dc.description.abstract The most striking characteristic of CHO cells is their adaptability, which enables efficient production of proteins as well as growth under a variety of culture conditions, but also results in genomic and phenotypic instability. To investigate the relative contribution of genomic and epigenetic modifications towards phenotype evolution, comprehensive genome and epigenome data are presented for six related CHO cell lines, both in response to perturbations (different culture conditions and media as well as selection of a specific phenotype with increased transient productivity) and in steady state (prolonged time in culture under constant conditions). Clear transitions were observed in DNA-methylation patterns upon each perturbation, while few changes occurred over time under constant conditions. Only minor DNA-methylation changes were observed between exponential and stationary growth phase; however, throughout a batch culture the histone modification pattern underwent continuous adaptation. Variation in genome sequence between the six cell lines on the level of SNPs, InDels, and structural variants is high, both upon perturbation and under constant conditions over time. The here presented comprehensive resource may open the door to improved control and manipulation of gene expression during industrial bioprocesses based on epigenetic mechanisms. Biotechnol. Bioeng. 2016;113: 2241–2253. © 2016 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc.
- dc.format.mimetype application/pdfca
- dc.identifier.citation Feichtinger J, Hernández I, Fischer C, Hanscho M, Auer N, Hackl M [et. al.]. Comprehensive genome and epigenome characterization of CHO cells in response to evolutionary pressures and over time. Biotechnology and Bioengineering. 2016; 113(10): 2241-2253. DOI: 10.1002/bit.25990ca
- dc.identifier.doi http://dx.doi.org/10.1002/bit.25990
- dc.identifier.issn 0006-3592ca
- dc.identifier.uri http://hdl.handle.net/10230/28079
- dc.language.iso engca
- dc.publisher Wileyca
- dc.relation.ispartof Biotechnology and Bioengineering. 2016; 113(10): 2241-2253
- dc.rights © 2016 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.ca
- dc.rights.accessRights info:eu-repo/semantics/openAccessca
- dc.rights.uri https://creativecommons.org/licenses/by/4.0/
- dc.subject.keyword Chinese hamster ovary cells
- dc.subject.keyword Epigenome
- dc.subject.keyword Genome variation
- dc.subject.keyword DNA-methylation
- dc.subject.keyword Histone modifications
- dc.title Comprehensive genome and epigenome characterization of CHO cells in response to evolutionary pressures and over timeca
- dc.type info:eu-repo/semantics/articleca
- dc.type.version info:eu-repo/semantics/publishedVersionca