Nanoporous graphene-based thin-film microelectrodes for in vivo high-resolution neural recording and stimulation

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  • dc.contributor.author Viana, Damià
  • dc.contributor.author Rodríguez Lucas, Elisa
  • dc.contributor.author Gener, Thomas
  • dc.contributor.author Puig, Maria Victoria
  • dc.contributor.author Garrido, Jose A.
  • dc.date.accessioned 2024-10-30T07:16:58Z
  • dc.date.available 2024-10-30T07:16:58Z
  • dc.date.issued 2024
  • dc.description.abstract One of the critical factors determining the performance of neural interfaces is the electrode material used to establish electrical communication with the neural tissue, which needs to meet strict electrical, electrochemical, mechanical, biological and microfabrication compatibility requirements. This work presents a nanoporous graphene-based thin-film technology and its engineering to form flexible neural interfaces. The developed technology allows the fabrication of small microelectrodes (25 µm diameter) while achieving low impedance (∼25 kΩ) and high charge injection (3-5 mC cm-2). In vivo brain recording performance assessed in rodents reveals high-fidelity recordings (signal-to-noise ratio >10 dB for local field potentials), while stimulation performance assessed with an intrafascicular implant demonstrates low current thresholds (<100 µA) and high selectivity (>0.8) for activating subsets of axons within the rat sciatic nerve innervating tibialis anterior and plantar interosseous muscles. Furthermore, the tissue biocompatibility of the devices was validated by chronic epicortical (12 week) and intraneural (8 week) implantation. This work describes a graphene-based thin-film microelectrode technology and demonstrates its potential for high-precision and high-resolution neural interfacing.
  • dc.description.sponsorship This work has been funded by the European Union Horizon 2020 Research and Innovation programme under grant agreement number 881603 (Graphene Core3); FLAG-ERA JTC 2021 project RESCUEGRAPH, from the Agencia Estatal de Investigación of Spain PCI2021-122075-2A y PCI2021-122095-2A financiados por MCIN/AEI/10.13039/501100011033 y por la Unión Europea NextGenerationEU/PRTR; TERCEL (RD12/0019/0011), CIBERNED (CB06/05/1105) and CIBER-BBN (CB06/01/0049) funds from the Instituto de Salud Carlos III of Spain, Proyecto PID2020-113663RB-I00 financiado por MCIN/AEI/10.13039/501100011033, from the Generalitat de Catalunya (2021SGR00495) and from the European Union’s Horizon Europe research and innovation programme under grant agreement number 101070865 (MINIGRAPH). The ICN2 is supported by the Severo Ochoa Centres of Excellence programme, Grant CEX2021-001214-S, funded by MCIN/AEI/10.13039.501100011033, and by the CERCA Programme/Generalitat de Catalunya. D.V. and S.M.-S. have been supported by the International PhD Programme La Caixa–Severo Ochoa (Programa Internacional de Becas ‘la Caixa’–Severo Ochoa). D.V. acknowledges that this work has been done in the framework of a PhD in electrical and telecommunication engineering at the Universitat Autònoma de Barcelona. J.d.V. acknowledges the Ministerio de Economía, Industria y Competitividad of Spain for Juan de la Cierva incorporation fellowship. M.C.S. received funding from the European Union Horizon 2020 under the Marie Sklodowska-Curie grant agreement number 754510 (PROBIST). E.d.C. acknowledges Ayuda RYC2019-027879-I financiada por MCIN/AEI/10.13039/501100011033 y por El FSE invierte en tu futuro. N.R. acknowledges Ayuda PRE2020-093708 financiada por MCIN/AEI/10.13039/501100011033 y por FSE invierte en tu futuro. E.M.-C. acknowledges Ayuda FJC2021-046601-I financiada por MCIN/AEI/10.13039/501100011033 y por la Unión Europea NextGenerationEU/PRTR. Authors acknowledge M. Ezcurdia for assistance in the bending experiments. S.M.-S and J.A. acknowledge funding from Generalitat de Catalunya 2021SGR00457. This study is part of the Advanced Materials programme and was supported by MCIN with funding from European Union NextGenerationEU (PRTR-C17.I1) and by Generalitat de Catalunya. This work has made use of the Spanish ICTS Network MICRONANOFABS partially supported by MICINN and the ICTS ‘NANBIOSIS’, more specifically by the Micro-NanoTechnology Unit of the CIBER-BBN at the IMB-CNM. Part of the present work has been performed in the framework of Universitat Autònoma de Barcelona Materials Science PhD programme and Neuroscience PhD programme. The focused ion beam sample preparation was conducted in the Laboratorio de Microscopias Avanzadas at Instituto de Nanociencia de Aragon-Universidad de Zaragoza. Authors acknowledge the LMA-INA for offering access to their instruments and expertise. This study was also financed by grant PID2019-104683RB-I00 funded by MCIN/AEI/10.13039/501100011033 to M.V.P. The authors would like to acknowledge P. Nebot for technical assistance. The authors would also like to thank C. Bullock and C. Bussy for their contributions during the early stages of the project. The University of Manchester Bioimaging and Single Cell Genomics Facility microscopes used in this study were purchased with grants from the UKRI Biotechnology and Biological Sciences Research Council (BBSRC), the Wellcome Trust and the University of Manchester Strategic Fund. The RT97 antibody was obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by the University of Iowa, Department of Biology.
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Viana D, Walston ST, Masvidal-Codina E, Illa X, Rodríguez-Meana B, Del Valle J, et al. Nanoporous graphene-based thin-film microelectrodes for in vivo high-resolution neural recording and stimulation. Nat Nanotechnol. 2024 Apr;19(4):514-23. DOI: 10.1038/s41565-023-01570-5
  • dc.identifier.doi http://dx.doi.org/10.1038/s41565-023-01570-5
  • dc.identifier.issn 1748-3387
  • dc.identifier.uri http://hdl.handle.net/10230/68394
  • dc.language.iso eng
  • dc.publisher Nature Research
  • dc.relation.ispartof Nat Nanotechnol. 2024 Apr;19(4):514-23
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/881603
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PID2020-113663RB-I00
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/HE/101070865
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/754510
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PID2019-104683RB-I00
  • dc.rights © The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri http://creativecommons.org/licenses/by/4.0/
  • dc.subject.keyword Bionanoelectronics
  • dc.subject.keyword Biomaterials
  • dc.title Nanoporous graphene-based thin-film microelectrodes for in vivo high-resolution neural recording and stimulation
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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