Proneural and mesenchymal glioma stem cells display major differences in splicing and lncRNA profiles

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• dc.contributor.author Guardia, Gabriela D. A.
• dc.contributor.author Correa, Bruna R.
• dc.contributor.author Araujo, Patricia Rosa
• dc.contributor.author Qiao, Mei
• dc.contributor.author Burns, Suzanne
• dc.contributor.author Penalva, Luis O. F.
• dc.contributor.author Galante, Pedro A. F.
• dc.date.accessioned 2020-04-27T10:21:46Z
• dc.date.available 2020-04-27T10:21:46Z
• dc.date.issued 2020
• dc.description.abstract Therapy resistance and recurrence in high-grade gliomas are driven by their populations of glioma stem cells (GSCs). Thus, detailed molecular characterization of GSCs is needed to develop more effective therapies. We conducted a study to identify differences in the splicing profile and expression of long non-coding RNAs in proneural and mesenchymal GSC cell lines. Genes related to cell cycle, DNA repair, cilium assembly, and splicing showed the most differences between GSC subgroups. We also identified genes distinctly associated with survival among patients of mesenchymal or proneural subgroups. We determined that multiple long non-coding RNAs with increased expression in mesenchymal GSCs are associated with poor survival of glioblastoma patients. In summary, our study established critical differences between proneural and mesenchymal GSCs in splicing profiles and expression of long non-coding RNA. These splicing isoforms and lncRNA signatures may contribute to the uniqueness of GSC subgroups, thus contributing to cancer phenotypes and explaining differences in therapeutic responses.
• dc.description.sponsorship This study was supported by a grant from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazil to PAFG and LOFP and by NIH 7R21CA175875-03. This study was partially supported by grants from Serrapilheira foundation and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP; 2018/15579-8) to PAFG. GDAG and BRC were supported by fellowships from FAPESP (2017/19541-2) and (2013/25483-4 and 2013/07159-5), respectively. PRA was supported by CPRIT Training Grant - RP140105
• dc.format.mimetype application/pdf
• dc.identifier.citation Guardia GDA, Correa BR, Araujo PR, Qiao M, Burns S, Penalva LOF et al. Proneural and mesenchymal glioma stem cells display major differences in splicing and lncRNA profiles. NPJ Genom Med. 2020 Jan 16; 5: 2. DOI: 10.1038/s41525-019-0108-5
• dc.identifier.doi http://dx.doi.org/10.1038/s41525-019-0108-5
• dc.identifier.issn 2056-7944
• dc.identifier.uri http://hdl.handle.net/10230/44342
• dc.language.iso eng
• dc.publisher Nature Research
• dc.relation.ispartof NPJ Genomic Medicine. 2020 Jan 16;5:2
• dc.rights © 2020 Gabriela D.A. Guardia et al. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.other Càncer
• dc.subject.other Genòmica
• dc.subject.other Genètica
• dc.title Proneural and mesenchymal glioma stem cells display major differences in splicing and lncRNA profiles
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion