Spatiotemporal transcriptomic atlas of mouse organogenesis using DNA nanoball-patterned arrays
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- dc.contributor.author Chen, Ao
- dc.contributor.author Muñoz Cánoves, Pura, 1962-
- dc.contributor.author Wang, Jian
- dc.date.accessioned 2022-06-17T05:51:52Z
- dc.date.available 2022-06-17T05:51:52Z
- dc.date.issued 2022
- dc.description.abstract Spatially resolved transcriptomic technologies are promising tools to study complex biological processes such as mammalian embryogenesis. However, the imbalance between resolution, gene capture, and field of view of current methodologies precludes their systematic application to analyze relatively large and three-dimensional mid- and late-gestation embryos. Here, we combined DNA nanoball (DNB)-patterned arrays and in situ RNA capture to create spatial enhanced resolution omics-sequencing (Stereo-seq). We applied Stereo-seq to generate the mouse organogenesis spatiotemporal transcriptomic atlas (MOSTA), which maps with single-cell resolution and high sensitivity the kinetics and directionality of transcriptional variation during mouse organogenesis. We used this information to gain insight into the molecular basis of spatial cell heterogeneity and cell fate specification in developing tissues such as the dorsal midbrain. Our panoramic atlas will facilitate in-depth investigation of longstanding questions concerning normal and abnormal mammalian development.
- dc.format.mimetype application/pdf
- dc.identifier.citation Chen A, Liao S, Cheng M, Ma K, Wu L, Lai Y et al. Spatiotemporal transcriptomic atlas of mouse organogenesis using DNA nanoball-patterned arrays. Cell. 2022 May 12;185(10):1777-92.e21. DOI: 10.1016/j.cell.2022.04.003
- dc.identifier.doi http://dx.doi.org/10.1016/j.cell.2022.04.003
- dc.identifier.issn 0092-8674
- dc.identifier.uri http://hdl.handle.net/10230/53512
- dc.language.iso eng
- dc.publisher Elsevier
- dc.relation.ispartof Cell. 2022 May 12;185(10):1777-92.e21
- dc.rights © 2022 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by/4.0/
- dc.subject.keyword Brain
- dc.subject.keyword Cell atlas
- dc.subject.keyword Cell differentiation
- dc.subject.keyword Cell lineages
- dc.subject.keyword Development
- dc.subject.keyword Developmental diseases
- dc.subject.keyword Mouse organogenesis
- dc.subject.keyword Progenitors
- dc.subject.keyword Single-cell
- dc.subject.keyword Spatial transcriptomics
- dc.title Spatiotemporal transcriptomic atlas of mouse organogenesis using DNA nanoball-patterned arrays
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion