Saturation of recognition elements blocks evolution of new tRNA identities

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dc.contributor.authorSaint-Léger, Adélaïdeca
dc.contributor.authorBello, Carla, 1990-ca
dc.contributor.authorDans, Pablo D.ca
dc.contributor.authorGabriel Torres, Adrianca
dc.contributor.authorNovoa, Eva Mariaca
dc.contributor.authorCamacho, Noeliaca
dc.contributor.authorOrozco, Modestoca
dc.contributor.authorKondrashov, Fyodor A., 1979-ca
dc.contributor.authorRibas de Pouplana, Lluísca
dc.date.accessioned2017-01-26T13:35:08Z
dc.date.available2017-01-26T13:35:08Z
dc.date.issued2016ca
dc.description.abstractUnderstanding the principles that led to the current complexity of the genetic code is a central question in evolution. Expansion of the genetic code required the selection of new transfer RNAs (tRNAs) with specific recognition signals that allowed them to be matured, modified, aminoacylated, and processed by the ribosome without compromising the fidelity or efficiency of protein synthesis. We show that saturation of recognition signals blocks the emergence of new tRNA identities and that the rate of nucleotide substitutions in tRNAs is higher in species with fewer tRNA genes. We propose that the growth of the genetic code stalled because a limit was reached in the number of identity elements that can be effectively used in the tRNA structure.
dc.description.sponsorshipThis work was supported in part by the Spanish Ministry of Economy and Competitiveness (grants BIO2012-32200, Sev-2012-0208, and BIO2012-32868 to L.R.d.P., F.A.K., and M.O., respectively) and by the Catalan Government (grants 2014-SGR-0771, 2014-SGR-0974, and 2014-SGR-0134 to L.R.d.P., F.A.K., and M.O., respectively). This work was also supported by the Howard Hughes Medical Institute International Early Career Scientist Program (55007424), by a European Research Council (ERC) Starting Grant (335980_EinME to F.K.), and by a grant from the ERC (ERC_SimDNA to M.O). A.G.T. and C.B. are funded by the Spanish Ministry of Economy and Competitiveness (FPDI-2013-17742 and BES-2013-064004, respectively).
dc.format.mimetypeapplication/pdfca
dc.identifier.citationSaint-Léger A, Bello Cabrera C, Dans PD, Gabriel Torres A, Novoa EM, Camacho N, Orozco M, Kondrashov FA, Ribas de Pouplana L. Saturation of recognition elements blocks evolution of new tRNA identities. Science Advances. 2016;2(4):e1501860. DOI: 10.1126/sciadv.1501860ca
dc.identifier.doihttp://dx.doi.org/10.1126/sciadv.1501860
dc.identifier.issn2375-2548ca
dc.identifier.urihttp://hdl.handle.net/10230/27999
dc.language.isoengca
dc.publisherAmerican Association for the Advancement of Science (AAAS)ca
dc.relation.ispartofScience Advances. 2016;2(4):e1501860
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/335980
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/3PN/BIO2012-32200
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/3PN/BIO2012-32868
dc.rights2016 © The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). 10.1126/sciadv.1501860ca
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject.keywordTRNA identities
dc.subject.keywordGenetic code
dc.subject.keywordEvolution
dc.subject.keywordADAT
dc.subject.keywordTRNA gly
dc.titleSaturation of recognition elements blocks evolution of new tRNA identitiesca
dc.typeinfo:eu-repo/semantics/articleca
dc.type.versioninfo:eu-repo/semantics/publishedVersionca

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