Surgical induction of endometriosis in female mice
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- dc.contributor.author Escudero Lara, Alejandra, 1992-
- dc.contributor.author Cabañero Ferri, David
- dc.contributor.author Maldonado, Rafael, 1961-
- dc.date.accessioned 2023-04-20T06:14:17Z
- dc.date.available 2023-04-20T06:14:17Z
- dc.date.issued 2020
- dc.description.abstract Endometriosis is a common gynecological disease characterized by the presence of endometrial tissue outside the uterine cavity. It is frequently associated with pain, infertility and a reduced quality of life, and it lacks adequate treatment. Several rodent models of endometriosis have been developed through heterologous and homologous transplantation of endometrial tissue into the abdominal compartment. Here we describe a surgical procedure to generate a syngeneic model of endometriosis in immunocompetent mice with intact uterine and ovarian tissues. In this model, four uterine fragments from a donor mouse at diestrus are sutured to the abdominal wall of a recipient mouse. One month after surgeries, endometrial implants develop into cysts with glandular epithelium and stroma, mimicking the endometriotic lesions observed in women with endometriosis. Therefore, this mouse model provides a valuable tool to study the pathophysiology of endometriosis and the efficacy of potential treatments.
- dc.description.sponsorship “Instituto de Salud Carlos III”, “Redes temáticas de investigación cooperativa en salud–Red de trastornos adictivos” (#RD16/0017/0020), “Ministerio de Ciencia, Innovación y Universidades”, MCIU (#SAF2017-84060-R-AEI/FEDER-UE), “Generalitat de Catalunya- Agència de Gestió d'Ajuts Universitaris i de Recerca-AGAUR" (#2017-SGR-669 and #ICREA Acadèmia2015) to R.M. are acknowledged.
- dc.format.mimetype application/pdf
- dc.identifier.citation Escudero-Lara A, Cabañero D, Maldonado R. Surgical induction of endometriosis in female mice. Bio Protoc. 2020 Sep 20;10(18):e3763. DOI: 10.21769/BioProtoc.3763
- dc.identifier.doi http://dx.doi.org/10.21769/BioProtoc.3763
- dc.identifier.issn 2331-8325
- dc.identifier.uri http://hdl.handle.net/10230/56512
- dc.language.iso eng
- dc.publisher Bio-protocol LLC
- dc.relation.ispartof Bio Protoc. 2020 Sep 20;10(18):e3763
- dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/SAF2017-84060-R
- dc.rights Copyright Escudero-Lara et al. This article is distributed under the terms of the Creative Commons Attribution License (CC BY 4.0).
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by/4.0/
- dc.subject.keyword Endometriosis
- dc.subject.keyword Female
- dc.subject.keyword Model
- dc.subject.keyword Mouse
- dc.subject.keyword Surgery
- dc.subject.keyword Syngeneic
- dc.title Surgical induction of endometriosis in female mice
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion