Single-cell transcriptome conservation in cryopreserved cells and tissues
Mostra el registre complet Registre parcial de l'ítem
- dc.contributor.author Guillaumet-Adkins, Amyca
- dc.contributor.author Rodríguez Esteban, Gustavoca
- dc.contributor.author Mereu, Elisabettaca
- dc.contributor.author Méndez Lago, Maríaca
- dc.contributor.author Jaitin, Diego Adhemarca
- dc.contributor.author Villanueva, Albertoca
- dc.contributor.author Vidal, Augustca
- dc.contributor.author Martínez Martí, Alexca
- dc.contributor.author Felip, Enriquetaca
- dc.contributor.author Vivancos Prellezo, Anaca
- dc.contributor.author Keren-Shaul, Hadasca
- dc.contributor.author Heath, Simonca
- dc.contributor.author Gut, Martaca
- dc.contributor.author Amit, Idoca
- dc.contributor.author Gut, Ivo Glynneca
- dc.contributor.author Heyn, Holgerca
- dc.date.accessioned 2018-05-31T07:32:26Z
- dc.date.available 2018-05-31T07:32:26Z
- dc.date.issued 2017
- dc.description.abstract A variety of single-cell RNA preparation procedures have been described. So far, protocols require fresh material, which hinders complex study designs. We describe a sample preservation method that maintains transcripts in viable single cells, allowing one to disconnect time and place of sampling from subsequent processing steps. We sequence single-cell transcriptomes from >1000 fresh and cryopreserved cells using 3'-end and full-length RNA preparation methods. Our results confirm that the conservation process did not alter transcriptional profiles. This substantially broadens the scope of applications in single-cell transcriptomics and could lead to a paradigm shift in future study designs.
- dc.format.mimetype application/pdf
- dc.identifier.citation Guillaumet-Adkins A, Rodríguez-Esteban G, Mereu E, Mendez-Lago M, Jaitin DA, Villanueva A et al. Single-cell transcriptome conservation in cryopreserved cells and tissues. Genome Biol. 2017 Mar 1;18(1):45. DOI: 10.1186/s13059-017-1171-9
- dc.identifier.doi http://dx.doi.org/10.1186/s13059-017-1171-9
- dc.identifier.issn 1474-7596
- dc.identifier.uri http://hdl.handle.net/10230/34756
- dc.language.iso eng
- dc.publisher BioMed Centralca
- dc.relation.ispartof Genome Biology. 2017 Mar 1;18(1):45
- dc.rights © The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by/4.0/
- dc.subject.keyword Conservation
- dc.subject.keyword Cryopreservation
- dc.subject.keyword Mars-seq
- dc.subject.keyword Pbmc
- dc.subject.keyword Pdox
- dc.subject.keyword Patient-derived orthotopic xenograft
- dc.subject.keyword Peripheral blood mononuclear cells
- dc.subject.keyword Rna sequencing
- dc.subject.keyword Single-cell genomics
- dc.subject.keyword Smart-seq2
- dc.subject.keyword Transcriptomics
- dc.title Single-cell transcriptome conservation in cryopreserved cells and tissuesca
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion