Using single-cell transcriptomics to understand functional states and interactions in microbial eukaryotes
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- dc.contributor.author Ku, Chuan
- dc.contributor.author Sebé-Pedrós, Arnau
- dc.date.accessioned 2020-04-02T10:20:15Z
- dc.date.issued 2019
- dc.description.abstract Understanding the diversity and evolution of eukaryotic microorganisms remains one of the major challenges of modern biology. In recent years, we have advanced in the discovery and phylogenetic placement of new eukaryotic species and lineages, which in turn completely transformed our view on the eukaryotic tree of life. But we remain ignorant of the life cycles, physiology and cellular states of most of these microbial eukaryotes, as well as of their interactions with other organisms. Here, we discuss how high-throughput genome-wide gene expression analysis of eukaryotic single cells can shed light on protist biology. First, we review different single-cell transcriptomics methodologies with particular focus on microbial eukaryote applications. Then, we discuss single-cell gene expression analysis of protists in culture and what can be learnt from these approaches. Finally, we envision the application of single-cell transcriptomics to protist communities to interrogate not only community components, but also the gene expression signatures of distinct cellular and physiological states, as well as the transcriptional dynamics of interspecific interactions. Overall, we argue that single-cell transcriptomics can significantly contribute to our understanding of the biology of microbial eukaryotes. This article is part of a discussion meeting issue 'Single cell ecology'.
- dc.format.mimetype application/pdf
- dc.identifier.citation Ku C, Sebé-Pedrós A. Using single-cell transcriptomics to understand functional states and interactions in microbial eukaryotes. Philos Trans R Soc Lond B Biol Sci. 2019; 374(1786):20190098. DOI: 10.1098/rstb.2019.0098
- dc.identifier.doi http://dx.doi.org/10.1098/rstb.2019.0098
- dc.identifier.issn 0962-8436
- dc.identifier.uri http://hdl.handle.net/10230/44148
- dc.language.iso eng
- dc.publisher Royal Society
- dc.relation.ispartof Philos Trans R Soc Lond B Biol Sci. 2019; 374(1786):20190098
- dc.rights © The Royal Society http://dx.doi.org/10.1098/rstb.2019.0098
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.subject.keyword Gene expression analysis
- dc.subject.keyword scRNA-seq
- dc.subject.keyword Environmental sampling
- dc.subject.keyword Protists
- dc.subject.keyword Unicellular eukaryotes
- dc.title Using single-cell transcriptomics to understand functional states and interactions in microbial eukaryotes
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/acceptedVersion