Centrosome clustering and cyclin D1 gene amplification in double minutes are common events in chromosomal

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• dc.contributor.author del Rey, Javierca
• dc.contributor.author Prat, Estherca
• dc.contributor.author Ponsa, Immaculadaca
• dc.contributor.author Lloreta, Josep, 1958-ca
• dc.contributor.author Gelabert, Antonica
• dc.contributor.author Algaba, Ferranca
• dc.contributor.author Camps-Puchadas, Jordica
• dc.contributor.author Miró, Rosaca
• dc.date.accessioned 2015-04-13T07:30:13Z
• dc.date.available 2015-04-13T07:30:13Z
• dc.date.issued 2010ca
• dc.description.abstract Background: Aneuploidy, centrosome abnormalities and gene amplification are hallmarks of chromosome instability (CIN) in cancer. Yet there are no studies of the in vivo behavior of these phenomena within the same bladder tumor. Methods: Twenty-one paraffin-embedded bladder tumors were analyzed by conventional comparative genome hybridization and fluorescence in situ hybridization (FISH) with a cyclin D1 gene (CCND1)/centromere 11 dual-color probe. Immunofluorescent staining of α, β and γ tubulin was also performed. Results: Based on the CIN index, defined as the percentage of cells not displaying the modal number for chromosome 11, tumors were classified as CIN-negative and CIN-positive. Fourteen out of 21 tumors were considered CIN-positive. All T1G3 tumors were included in the CIN-positive group whereas the majority of Ta samples were classified as CIN-negative tumors. Centrosome clustering was observed in six out of 12 CIN-positive tumors analyzed. CCND1 amplification in homogeneously staining regions was present in six out of 14 CIN-positive tumors; three of them also showed amplification of this gene in double minutes. Conclusions: Complex in vivo behavior of CCND1 amplicon in bladder tumor cells has been demonstrated by accurate FISH analysis on paraffin-embedded tumors. Positive correlation between high heterogeneity, centrosome abnormalities and CCND1 amplification was found in T1G3 bladder carcinomas. This is the first study to provide insights into the coexistence of CCND1 amplification in homogeneously staining regions and double minutes in primary bladder tumors. It is noteworthy that those patients whose tumors showed double minutes had a significantly shorter overall survival rate (p < 0.001).en
• dc.description.sponsorship The work has been supported by ISCIII: EPICUR-Red (FIS G03/174), SAF2007-64167 and RD06/0020/1020. Javier del Rey was a fellow of Generalitat de Catalunya and is supported by RD06/0020/1020en
• dc.format.mimetype application/pdfca
• dc.identifier.citation del Rey J, Prat E, Ponsa I, Lloreta J, Gelabert A, Algaba F, et al. Centrosome clustering and cyclin D1 gene amplification in double minutes are common events in chromosomal. BMC Cancer. 2010;10:280. DOI: 10.1186/1471-2407-10-280ca
• dc.identifier.doi http://dx.doi.org/10.1186/1471-2407-10-280
• dc.identifier.issn 1471-2407ca
• dc.identifier.uri http://hdl.handle.net/10230/23397
• dc.language.iso engca
• dc.publisher BioMed Centralca
• dc.relation.ispartof BMC Cancer. 2010;10:280
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PN/SAF2007-64167
• dc.rights © del Rey J, Prat E, Ponsa I, Lloreta J, Gelabert A, Algaba F, Camps J, Miró R. Creative Commons Attribution License http://creativecommons.org/licenses/by/2.0/ca
• dc.rights.accessRights info:eu-repo/semantics/openAccessca
• dc.rights.uri http://creativecommons.org/licenses/by/2.0
• dc.subject.other Bufeta -- Càncerca
• dc.subject.other Carcinogènesica
• dc.subject.other Mitosica
• dc.title Centrosome clustering and cyclin D1 gene amplification in double minutes are common events in chromosomalen
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/publishedVersionca