Genome sequence of the necrotrophic fungus Penicillium digitatum, the main postharvest pathogen of citrus

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  • dc.contributor.author Marcet Houben, Marinaca
  • dc.contributor.author Ballester, Ana Rosaca
  • dc.contributor.author de la Fuente, Beatrizca
  • dc.contributor.author Harries, Eleonoraca
  • dc.contributor.author Marcos del Águila, Josep, 1971-ca
  • dc.contributor.author González Candelas, Luisca
  • dc.contributor.author Gabaldón Estevan, Juan Antonio, 1973-ca
  • dc.date.accessioned 2015-03-16T08:15:35Z
  • dc.date.available 2015-03-16T08:15:35Z
  • dc.date.issued 2012ca
  • dc.description.abstract Background: Penicillium digitatum is a fungal necrotroph causing a common citrus postharvest disease known as green mold. In order to gain insight into the genetic bases of its virulence mechanisms and its high degree of host-specificity, the genomes of two P. digitatum strains that differ in their antifungal resistance traits have been sequenced and compared with those of 28 other Pezizomycotina. Results: The two sequenced genomes are highly similar, but important differences between them include the presence of a unique gene cluster in the resistant strain, and mutations previously shown to confer fungicide resistance. The two strains, which were isolated in Spain, and another isolated in China have identical mitochondrial genome sequences suggesting a recent worldwide expansion of the species. Comparison with the closely-related but non-phytopathogenic P. chrysogenum reveals a much smaller gene content in P. digitatum, consistent with a more specialized lifestyle. We show that large regions of the P. chrysogenum genome, including entire supercontigs, are absent from P. digitatum, and that this is the result of large gene family expansions rather than acquisition through horizontal gene transfer. Our analysis of the P. digitatum genome is indicative of heterothallic sexual reproduction and reveals the molecular basis for the inability of this species to assimilate nitrate or produce the metabolites patulin and penicillin. Finally, we identify the predicted secretome, which provides a first approximation to the protein repertoire used during invasive growth. Conclusions: The complete genome of P. digitatum, the first of a phytopathogenic Penicillium species, is a valuable tool for understanding the virulence mechanisms and host-specificity of this economically important pest.en
  • dc.description.sponsorship TG research is funded in part by a grant from the Spanish Ministry of Economy and Innovation (BFU2009-09168). JFM research is funded in part by the Spanish Ministry of Science (BIO2009-12919). LGC research is funded in part by the Spanish Ministry of Economy and Innovation (AGL-2008-04828-C03-02 and AGL2011-30519-C03-01) and the Generalitat Valenciana (PROMETEO/2010/010 and ACOMP/2011/250). ARB acknowledges the support of the JAE-Doc program from CSIC and the European FEDER fundsen
  • dc.format.mimetype application/pdfca
  • dc.identifier.citation Marcet-Houben M, Ballester AR, de la Fuente B, Harries E, Marcos JF, González-Candelas L et al. Genome sequence of the necrotrophic fungus Penicillium digitatum, the main postharvest pathogen of citrus. BMC Genomics. 2012; 13: 646. DOI 10.1186/1471-2164-13-646ca
  • dc.identifier.doi http://dx.doi.org/10.1186/1471-2164-13-646
  • dc.identifier.issn 1471-2164ca
  • dc.identifier.uri http://hdl.handle.net/10230/23179
  • dc.language.iso engca
  • dc.publisher BioMed Centralca
  • dc.relation.ispartof BMC Genomics. 2012; 13: 646
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2009-09168
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BIO2009-12919
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/AGL2008-04828
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/AGL2011-30519
  • dc.rights © 2012 Marcet-Houben et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en
  • dc.rights.accessRights info:eu-repo/semantics/openAccessca
  • dc.rights.uri http://creativecommons.org/licenses/by/2.0
  • dc.subject.other Fongs -- Genèticaca
  • dc.subject.other Plantes -- Malaltiesca
  • dc.subject.other ADN -- Anàlisica
  • dc.title Genome sequence of the necrotrophic fungus Penicillium digitatum, the main postharvest pathogen of citrusen
  • dc.type info:eu-repo/semantics/articleca
  • dc.type.version info:eu-repo/semantics/publishedVersionca

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