The hologenome of Daphnia magna reveals possible DNA methylation and microbiome-mediated evolution of the host genome

Chaturvedi, Anurag; Li, Xiaojing; Dhandapani, Vignesh; Marshall, Hollie; Kissane, Stephen; Cuenca-Cambronero, Maria; Asole, Giovanni; Calvet, Ferriol; Ruiz-Romero, Marina; Marangio, Paolo; Guigó Serra, Roderic; Rago, Daria; Mirbahai, Leda; Eastwood, Niamh; Colbourne, John K.; Zhou, Jiarui; Mallon, Eamonn; Orsini, Luisa

doi:http://dx.doi.org/10.1093/nar/gkad685

The hologenome of Daphnia magna reveals possible DNA methylation and microbiome-mediated evolution of the host genome

Citation

Chaturvedi A, Li X, Dhandapani V, Marshall H, Kissane S, Cuenca-Cambronero M, Asole G, Calvet F, Ruiz-Romero M, Marangio P, Guigó R, Rago D, Mirbahai L, Eastwood N, Colbourne JK, Zhou J, Mallon E, Orsini L. The hologenome of Daphnia magna reveals possible DNA methylation and microbiome-mediated evolution of the host genome. Nucleic Acids Res. 2023 Oct 13;51(18):9785-803. DOI: 10.1093/nar/gkad685

Abstract

Properties that make organisms ideal laboratory models in developmental and medical research are often the ones that also make them less representative of wild relatives. The waterflea Daphnia magna is an exception, by both sharing many properties with established laboratory models and being a keystone species, a sentinel species for assessing water quality, an indicator of environmental change and an established ecotoxicology model. Yet, Daphnia's full potential has not been fully exploited because of the challenges associated with assembling and annotating its gene-rich genome. Here, we present the first hologenome of Daphnia magna, consisting of a chromosomal-level assembly of the D. magna genome and the draft assembly of its metagenome. By sequencing and mapping transcriptomes from exposures to environmental conditions and from developmental morphological landmarks, we expand the previously annotates gene set for this species. We also provide evidence for the potential role of gene-body DNA-methylation as a mutagen mediating genome evolution. For the first time, our study shows that the gut microbes provide resistance to commonly used antibiotics and virulence factors, potentially mediating Daphnia's environmental-driven rapid evolution. Key findings in this study improve our understanding of the contribution of DNA methylation and gut microbiota to genome evolution in response to rapidly changing environments.