Optimization of a liquid chromatography-tandem mass spectrometry method for the quantification of traces of taxanes in a Corylus avellana cell suspension medium

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• dc.contributor.author Gallego Palacios, Ana, 1987-ca
• dc.contributor.author Jáuregui, Olgaca
• dc.contributor.author Moyano Claramunt, Elisabetca
• dc.contributor.author Palazón, Javierca
• dc.contributor.author Casals, Isidreca
• dc.contributor.author Bonfill Baldrich, Ma. Mercedesca
• dc.date.accessioned 2016-04-27T13:23:37Z
• dc.date.available 2016-04-27T13:23:37Z
• dc.date.issued 2015
• dc.description.abstract Since the recent discovery of taxol and other taxanes in Corylus avellana, this plant species has attracted interest as a potential new source of these compounds. However, its low taxane content in comparison with Taxus spp. has restricted research to analytical identification or global quantitation. A feasible and sensitive method based on liquid chromatography-tandem mass spectrometry using a triple quadrupole analyzer was developed for the analysis of taxol and four other taxanes in a Corylus avellana cell suspension medium. Taxanes were extracted from the cell culture medium with dichloromethane and analyzed using electrospray ionization and quantified by multiple-reaction monitoring mode. Methanol and matrix-matching calibration curves using docetaxel as the internal standard were analyzed. Linearity was confirmed over the whole calibration range (0.3–2.1 μg mL−1). The inter- and intra-day precision of taxanes ranged from 80% to 120% and the recovery rates were higher than 80%. Limits of detection were between 0.24–38 ng mL−1 and the limits of quantification were between 0.8–125 ng mL−1. The low detection and quantitation values obtained allowed us to detect small quantities of the released taxanes (120 ng mL−1 of B, 151 ng mL−1 of CF and 105 ng mL−1 of T), which correspond to about 0.5 ng mL−1 of each taxane, in the 20 mL Corylus avellana cell suspension culture medium extracted, even at the beginning of the culture. These results were confirmed by high resolution mass spectrometry.ca
• dc.description.sponsorship This research has been supported by a grant from the Spanish MEC (BIO2011-29856-CO2-01) and a grant from the Catalan Government (2014SGR215). A. Gallego was supported by a fellowship from the University Pompeu Fabra.
• dc.format.mimetype application/pdfca
• dc.identifier.citation Gallego A, Jáuregui O, Moyano E, Palazón J, Casals I, Bonfill M. Optimization of a liquid chromatography-tandem mass spectrometry method for the quantification of traces of taxanes in a Corylus avellana cell suspension medium. RSC Advances. 2015;5:17976-83. DOI: 10.1039/C5RA00803Dca
• dc.identifier.doi http://dx.doi.org/10.1039/C5RA00803D
• dc.identifier.issn 2046-2069
• dc.identifier.uri http://hdl.handle.net/10230/26188
• dc.language.iso engca
• dc.publisher Royal Society of Chemistryca
• dc.relation.ispartof RSC Advances. 2015;5:17976-83
• dc.rights This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.ca
• dc.rights.accessRights info:eu-repo/semantics/openAccessca
• dc.rights.uri http://creativecommons.org/licenses/by/3.0/ca
• dc.subject.other Avellanerca
• dc.subject.other Plantes Cèl·lules i teixitsca
• dc.title Optimization of a liquid chromatography-tandem mass spectrometry method for the quantification of traces of taxanes in a Corylus avellana cell suspension mediumca
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/publishedVersionca