Insights into the Mechanisms of Basal Coordination of Transcription Using a Genome-Reduced Bacterium

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• dc.contributor.author Junier, Ivanca
• dc.contributor.author Unal, E. Besrayca
• dc.contributor.author Yus, Evaca
• dc.contributor.author Lloréns Rico, Verónica, 1989-ca
• dc.contributor.author Serrano Pubull, Luis, 1982-ca
• dc.date.accessioned 2017-04-19T07:21:16Z
• dc.date.available 2017-04-19T07:21:16Z
• dc.date.issued 2016
• dc.description.abstract Coordination of transcription in bacteria occurs at supra-operonic scales, but the extent, specificity, and mechanisms of such regulation are poorly understood. Here, we tackle this problem by profiling the transcriptome of the model organism Mycoplasma pneumoniae across 115 growth conditions. We identify three qualitatively different levels of co-expression corresponding to distinct relative orientations and intergenic properties of adjacent genes. We reveal that the degree of co-expression between co-directional adjacent operons, and more generally between genes, is tightly related to their capacity to be transcribed en bloc into the same mRNA. We further show that this genome-wide pervasive transcription of adjacent genes and operons is specifically repressed by DNA regions preferentially bound by RNA polymerases, by intrinsic terminators, and by large intergenic distances. Taken together, our findings suggest that the basal coordination of transcription is mediated by the physical entities and mechanical properties of the transcription process itself, and that operon-like behaviors may strongly vary from condition to condition.
• dc.description.sponsorship This work was supported by Fundación Marcelino Botin and the Spanish Ministerio de Economía y Competitividad (BIO2007-61762). This project was financed by Instituto de Salud Carlos III and co-financed by Federación Española de Enfermedades Raras under grant agreement PI10/01702 and the European Research Council and European Union’s Horizon 2020 research and innovation program under grant agreements 634942 (MycoSynVac) and 670216 (MYCOCHASSIS). The Centre for Genomic Regulation acknowledges the support of the Spanish Ministry of Economy and Competitiveness, “Centro de Excelencia Severo Ochoa 2013-2017,” SEV-2012-0208.
• dc.format.mimetype application/pdfca
• dc.identifier.citation Junier I, Unal EB, Yus E, Lloréns Rico V, Serrano Pubull L. Insights into the Mechanisms of Basal Coordination of Transcription Using a Genome-Reduced Bacterium. Cell Systems. 2016;2(6):391-401. DOI: 10.1016/j.cels.2016.04.015
• dc.identifier.doi http://dx.doi.org/10.1016/j.cels.2016.04.015
• dc.identifier.issn 2405-4720
• dc.identifier.uri http://hdl.handle.net/10230/30836
• dc.language.iso eng
• dc.publisher Elsevierca
• dc.relation.ispartof Cell Systems. 2016;2(6):391-401
• dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/634942
• dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/670216
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/SEV2012-0208
• dc.rights Cell Systems 2, 391–401, June 22, 2016. © 2016 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
• dc.title Insights into the Mechanisms of Basal Coordination of Transcription Using a Genome-Reduced Bacteriumca
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion