Dissecting the energy metabolism in Mycoplasma pneumoniae through genome-scale metabolic modeling
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- dc.contributor.author Wodke, Judith A.H.ca
- dc.contributor.author Puchalka, Jacekca
- dc.contributor.author Lluch-Senar, Maria 1982-ca
- dc.contributor.author Marcos del Águila, Josep, 1971-ca
- dc.contributor.author Yus, Evaca
- dc.contributor.author Godinho, Miguelca
- dc.contributor.author Gutiérrez Gallego, Ricardo, 1968-ca
- dc.contributor.author Martins dos Santos, Vitorca
- dc.contributor.author Serrano Pubull, Luis, 1982-ca
- dc.contributor.author Klipp, E., 1965-ca
- dc.contributor.author Maier, Tobiasca
- dc.date.accessioned 2014-04-29T08:44:39Z
- dc.date.available 2014-04-29T08:44:39Z
- dc.date.issued 2013ca
- dc.description.abstract Mycoplasma pneumoniae, a threatening pathogen with a minimal genome, is a model organism for bacterial systems biology for which substantial experimental information is available. With the goal of understanding the complex interactions underlying its metabolism, we analyzed and characterized the metabolic network of M. pneumoniae in great detail, integrating data from different omics analyses under a range of conditions into a constraint-based model backbone. Iterating model predictions, hypothesis generation, experimental testing, and model refinement, we accurately curated the network and quantitatively explored the energy metabolism. In contrast to other bacteria, M. pneumoniae uses most of its energy for maintenance tasks instead of growth. We show that in highly linear networks the prediction of flux distributions for different growth times allows analysis of time-dependent changes, albeit using a static model. By performing an in silico knock-out study as well as analyzing flux distributions in single and double mutant phenotypes, we demonstrated that the model accurately represents the metabolism of M. pneumoniae. The experimentally validated model provides a solid basis for understanding its metabolic regulatory mechanisms.
- dc.description.sponsorship This work was supported by the European Research council (ERC) advanced grant, the Fundacion Marcelino Botin and the Spanish Ministry of Research and Innovation to the ICREA researcher LS. JW is recipient of a la Caixa-CRG fellowship
- dc.format.mimetype application/pdfca
- dc.identifier.citation Wodke JA, Puchałka J, Lluch-Senar M, Marcos J, Yus E, Godinho M et al. Dissecting the energy metabolism in Mycoplasma pneumoniae through genome-scale metabolic modeling. Mol Syst Biol. 2013; 9: 653. DOI: 10.1038/msb.2013.6ca
- dc.identifier.doi http://dx.doi.org/10.1038/msb.2013.6
- dc.identifier.issn 1744-4292ca
- dc.identifier.uri http://hdl.handle.net/10230/22230
- dc.language.iso engca
- dc.publisher Nature Publishing Groupca
- dc.relation.ispartof Molecular Systems Biology. 2013;9:653
- dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/232913
- dc.rights This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike Licenseca
- dc.rights.accessRights info:eu-repo/semantics/openAccessca
- dc.rights.uri http://creativecommons.org/licenses/by/3.0/
- dc.subject.other Microorganismes patògens
- dc.subject.other Genètica
- dc.subject.other Genòmica
- dc.subject.other Metabolisme energètic
- dc.title Dissecting the energy metabolism in Mycoplasma pneumoniae through genome-scale metabolic modelingca
- dc.type info:eu-repo/semantics/articleca
- dc.type.version info:eu-repo/semantics/publishedVersion