The Hog1 SAPK controls the Rtg1/Rtg3 transcriptional complex activity by multiple regulatory mechanisms
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- dc.contributor.author Ruiz Roig, Clàudiaca
- dc.contributor.author Noriega Esteban, Núriaca
- dc.contributor.author Duch, Albaca
- dc.contributor.author Posas Garriga, Francescca
- dc.contributor.author Nadal Clanchet, Eulàlia deca
- dc.date.accessioned 2015-12-11T14:57:25Z
- dc.date.available 2015-12-11T14:57:25Z
- dc.date.issued 2012
- dc.description.abstract Cells modulate expression of nuclear genes in response to alterations in mitochondrial function, a response termed retrograde (RTG) regulation. In budding yeast, the RTG pathway relies on Rtg1 and Rtg3 basic helix-loop-helix leucine Zipper transcription factors. Exposure of yeast to external hyperosmolarity activates the Hog1 stress-activated protein kinase (SAPK), which is a key player in the regulation of gene expression upon stress. Several transcription factors, including Sko1, Hot1, the redundant Msn2 and Msn4, and Smp1, have been shown to be directly controlled by the Hog1 SAPK. The mechanisms by which Hog1 regulates their activity differ from one to another. In this paper, we show that Rtg1 and Rtg3 transcription factors are new targets of the Hog1 SAPK. In response to osmostress, RTG-dependent genes are induced in a Hog1-dependent manner, and Hog1 is required for Rtg1/3 complex nuclear accumulation. In addition, Hog1 activity regulates Rtg1/3 binding to chromatin and transcriptional activity. Therefore Hog1 modulates Rtg1/3 complex activity by multiple mechanisms in response to stress. Overall our data suggest that Hog1, through activation of the RTG pathway, contributes to ensure mitochondrial function as part of the Hog1-mediated osmoadaptive response.ca
- dc.description.sponsorship This work was supported by the Botin Foundation and grants from the Spanish Ministry of Science and Innovation (BFU2011-26722 to E.d.N. and BIO2009-07762 to F.P.) and the FP7 (UNICELLSYS) framework program.
- dc.format.mimetype application/pdfca
- dc.identifier.citation Ruiz-Roig C, Noriega N, Duch A, Posas F, de Nadal E. The Hog1 SAPK controls the Rtg1/Rtg3 transcriptional complex activity by multiple regulatory mechanisms. Molecular biology of the cell. 2012;23(21):4286-96. DOI 10.1091/mbc.E12-04-0289ca
- dc.identifier.doi http://dx.doi.org/10.1091/mbc.E12-04-0289
- dc.identifier.issn 1059-1524
- dc.identifier.uri http://hdl.handle.net/10230/25391
- dc.language.iso engca
- dc.publisher American Society for Cell Biologyca
- dc.relation.ispartof Molecular biology of the cell. 2012;23(21):4286-96
- dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/201142
- dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2011-26722
- dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BIO2009-07762
- dc.rights © 2012 Ruiz et al. This article is under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons Licenseca
- dc.rights.accessRights info:eu-repo/semantics/openAccessca
- dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/3.0ca
- dc.subject.other Saccharomyces cerevisiae -- Metabolismeca
- dc.subject.other Regulació genèticaca
- dc.title The Hog1 SAPK controls the Rtg1/Rtg3 transcriptional complex activity by multiple regulatory mechanismsca
- dc.type info:eu-repo/semantics/articleca
- dc.type.version info:eu-repo/semantics/publishedVersionca